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Bacteria, and in particular marine bacteria, can be found in environments that are poor in nutrients. To survive, they are able to move toward more favorable niches by a mechanism called chemotaxis, whose first step consists in the detection of substrates by chemoreceptors. We developed a chemotactic assay enabling rapid testing of several hundred different solutes and we identified several molecules eliciting a chemotactic response from two aquatic Shewanella species. We propose that this assay be used for other bacteria to determine the repertoire of chemotactic molecules, generally not clearly elucidated.
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http://dx.doi.org/10.1016/j.resmic.2011.03.001 | DOI Listing |
Elife
August 2025
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, United States.
Previously, we showed Enterobacteriaceae use chemotaxis and the chemoreceptor Tsr for attraction to blood serum (Glenn et al., 2024). Here, we investigated the complementary role of Tsr in mediating chemorepulsion, a behaviour by which motile bacteria avoid deleterious compounds to locate permissive niches.
View Article and Find Full Text PDFPlast Reconstr Surg
May 2025
From the Division of Plastic Surgery, Department of Surgery, Northwestern University-Feinberg School of Medicine.
Background: Women with cosmetic implants have lower rates of future breast cancer than the general population. The authors hypothesized that the implant foreign body response could induce a local protective anticancer immunosurveillance. The authors expanded on their previous finding, which showed that women with breast implants have elevated antibody responses to certain breast cancer proteins.
View Article and Find Full Text PDFSci Rep
March 2025
Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Upon their arrival in the water column, coral larvae use physical and chemical cues to navigate toward a suitable habitat and begin their settlement process. To engineer substrates that influence settlement, it is important to have quantitative data about the types and concentrations of chemicals that elicit desired behavioral responses before and after contact with the substrate. Here, we quantified the behavioral and morphological responses of coral larvae (Colpophyllia natans and Orbicella faveolata) to crustose coralline algae exudates (CCA) and ions found in coral skeletons using chemotactic assays in microfluidic channels.
View Article and Find Full Text PDFExp Cell Res
April 2025
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Mexico City, Mexico. Electronic address:
Calcium-sensing receptor (CaSR), a G protein-coupled receptor, is overexpressed in certain breast cancer tumors where it drives cell migration and secretion of chemotactic agonists, likely contributing to metastatic dissemination. Since CaSR activates breast cancer cell migration via the Gβγ-PI3K-mTORC2/Rac-1 pathway, we hypothesized that PKCζ and perhaps other protein kinase C (PKC) isoforms, known as mTORC2-regulated effectors, are involved in migratory and invasive signaling elicited by CaSR. We analyzed the effect of PKC inhibitors and siRNAs which pointed to PKCζ as effector of CaSR in cell migration and invasion.
View Article and Find Full Text PDFinfects human upper airways and deploys an array of immunosuppressive virulence factors, among which the adenylate cyclase toxin (CyaA) plays a prominent role in disarming host phagocytes. CyaA binds the complement receptor-3 (CR3 aka αβ integrin CD11b/CD18 or Mac-1) of myeloid cells and delivers into their cytosol an adenylyl cyclase enzyme that hijacks cellular signaling through unregulated conversion of cytosolic ATP to cAMP. We found that the action of as little CyaA as 22 pM (4 ng/mL) blocks macrophage colony-stimulating factor (M-CSF)-driven transition of migratory human CD14 monocytes into macrophages.
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