The chemical-in-μwell: a high-throughput technique for identifying solutes eliciting a chemotactic response in motile bacteria.

Res Microbiol

Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-UPR 9043, 31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France.

Published: November 2011


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Article Abstract

Bacteria, and in particular marine bacteria, can be found in environments that are poor in nutrients. To survive, they are able to move toward more favorable niches by a mechanism called chemotaxis, whose first step consists in the detection of substrates by chemoreceptors. We developed a chemotactic assay enabling rapid testing of several hundred different solutes and we identified several molecules eliciting a chemotactic response from two aquatic Shewanella species. We propose that this assay be used for other bacteria to determine the repertoire of chemotactic molecules, generally not clearly elucidated.

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http://dx.doi.org/10.1016/j.resmic.2011.03.001DOI Listing

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