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Applicability of RNA standards for evaluating RT-qPCR assays and platforms. | LitMetric

Applicability of RNA standards for evaluating RT-qPCR assays and platforms.

BMC Genomics

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK.

Published: February 2011


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Article Abstract

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052187PMC
http://dx.doi.org/10.1186/1471-2164-12-118DOI Listing

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