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Article Abstract
In this study we report the identification and characterization of channel catfish, Ictalurus punctatus CD8α and CD8β genes. Both genes encode predicted proteins containing a leader, a immunoglobulin superfamily V domain, a stalk/hinge region, a transmembrane region and a positively charged cytoplasmic tail (CYT) containing the conserved teleost C-X-H motif. Catfish CD8α and CD8β are encoded as single copy genes and as in other vertebrates exhibit a conserved head to tail synteny; the CD8β gene is found 14.1kb upstream of the CD8α gene. Both CD8α and CD8β transcripts showed a low degree of polymorphism. Finally, as determined by q-PCR both CD8α and CD8β are expressed in various catfish lymphoid tissues with the highest expression observed in thymus from 2 month old catfish-fry. In the future these results will provide the basis for evaluating the role of CD8(+) CTL and other CD8-bearing cells in response to immunization or infection in the catfish.
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| http://dx.doi.org/10.1016/j.fsi.2011.01.011 | DOI Listing |
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