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Michaelis-Menten kinetics of RasGAP proteins by a rapid fluorescence-based assay.
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Department of Pharmacology, Yale University, New Haven, CT 06510, USA; Yale Cancer Center, Yale University, New Haven, CT 06510, USA. Electronic address:
Ras small GTPases are essential for a wide range of cellular processes. These proteins cycle between the GDP-loaded and GTP-loaded states, and the actions of GTPase activating proteins (GAPs) are necessary to stimulate Ras-mediated GTP hydrolysis. Here, we provide a protocol to achieve Michaelis-Menten kinetic profiling of GAP-mediated stimulation of a small GTPase by real-time monitoring of inorganic phosphate release in vitro.
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Department and Graduate Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan. Electronic address:
Background: Atherosclerosis, a leading cause of cardiovascular disease (CVD) mortality worldwide, is characterized by dysregulated lipid metabolism and unresolved inflammation. Macrophage-derived foam cell formation and apoptosis contribute to plaque formation and vulnerability. Elevated serum galectin-3 (Gal-3) levels are associated with increased CVD risk, and Gal-3 in plaques is strongly associated with macrophages.
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Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
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IL-17A is a pro-inflammatory cytokine that significantly contributes to the pathogenesis of autoimmune diseases, including multiple sclerosis (MS). Previous studies have suggested that PARP-1 inhibitors can modulate IL-17A-mediated inflammation, prompting the investigation of Niraparib, an FDA-approved PARP-1 inhibitor, as a potential therapeutic agent for MS. In this study, we hypothesized that Niraparib could disrupt the interaction between IL-17A and its receptor, IL-17RA.
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Department of Obstetrics and Gynaecology, IRCCS San Raffaele Scientific Institute, 20132, Milan, Italy.
Objectives: Recommendations regarding the use of third-trimester ultrasound lack universal consensus. Yet, there is evidence which supports its value in assessing fetal growth, fetal well-being, and a number of pregnancy-related complications. This literature review evaluates the available scientific evidence regarding its applications, usefulness, and the timing of the third-trimester scan in a low-risk population.
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