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Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analyses showed the best correlation between data when an >or=80% cutoff was applied to MLVA. We confirmed the validity of MLVA for identification of related strains in a polyclonal MRSA population.
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http://dx.doi.org/10.1128/JCM.02220-09 | DOI Listing |
Comp Immunol Microbiol Infect Dis
August 2025
Institute of Veterinary Sciences, University of Blida 1, Algeria; Laboratory of Physical Chemistry of Material Interfaces Applied to the Environment, Saâd Dahlab University, Blida 1, B.P. 270, Route de Soumaa, Blida 09000, Algeria. Electronic address:
This systematic review, conducted according to PRISMA guidelines, provides a comprehensive synthesis of epidemiological data on Coxiella burnetii, the causative agent of Q fever, in humans and animals worldwide. A total of 1321 publications were identified, of which 166 met the inclusion criteria. Q fever, first described in 1937 in Australian abattoir workers, remains a globally distributed zoonosis, with domestic ruminants (sheep, goats, cattle) serving as the primary reservoirs and sources of human infection.
View Article and Find Full Text PDFSci Rep
August 2025
Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Erwinia amylovora is a highly destructive phytopathogenic bacterium that causes fire blight in Rosaceae plants, particularly apple and pear trees. Due to its status as a quarantine organism in many countries, extensive epidemiological and genotypic studies have been conducted to better understand and manage this pathogen. This study presents a novel workflow for identifying strain-specific simple sequence repeats (SSRs) in bacterial genomes, focusing on E.
View Article and Find Full Text PDFMol Biol Rep
July 2025
Bovine Tuberculosis Laboratory, Tuberculin and Mallein Research & Production Department, Agricultural Research, Education and Extension Organization (AREEO), Razi Vaccine and Serum Research Institute, Karaj, 3197619751, Iran.
Background: The multiplicity of infection sources along with the genetically homogenous nature of Mycobacterium avium subsp. paratuberculosis (MAP) necessitates the application of high-discriminative fingerprinting techniques to establish epidemiological links and control of paratuberculosis in ruminants. The present study aimed to develop a simple Multiple-Locus Variable-Number Tandem-Repeat Analysis (MLVA) assay suitable for low-budget laboratory settings.
View Article and Find Full Text PDFPLoS One
July 2025
Inflammatory Bowel Disease Research Laboratory, Canberra Hospital, Canberra, Australian Capital Territory, Australia.
Several studies report the diversity, and population structure of Escherichia coli (E. coli) in the human gut, but most used faecal specimens as the source of E. coli for analysis.
View Article and Find Full Text PDFAnimals (Basel)
May 2025
College of Veterinary Medicine & Institute for Veterinary Biomedical Science, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
Q fever, caused by , is a zoonotic disease that poses significant risks to both livestock productivity and human health. This is the first molecular and epidemiological study of infections on a Boer goat farm in South Korea, encompassing clinical cases in animals and humans, as well as environmental contamination. The investigation was initiated following abortion cases at a farm that co-raised Boer goats and cattle.
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