Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A disulfide-carrying polymer with many pendent mannose residues (dithiolated poly(2-methacryloyloxyethyl D-mannopyranoside)) was obtained by atom transfer radical polymerization (ATRP) method using 2-(2'-bromoisobutyroyloxy)ethyl disulfide as initiator. The obtained disulfide-carrying glycopolymer (DT-PMEMan) was accumulated as a polymer brush on a colloidal gold-immobilized glass substrate, and the binding processes of a lectin, Concanavalin A (Con A), to mannose residues in the polymer brush were examined using a UV-vis spectrophotometer with the help of a localized surface plasmon resonance. Con A showed a concentration-dependent specific binding to the glycopolymer brush, and the apparent association constant of Con A with mannose residues in the brush was much larger than the association constant for small sugars due to the so-called cluster effect. Furthermore, the glycopolymer brush-coated device had a detection limit lower than 5 nM. The glycopolymer-carrying device examined here is expected to expand our knowledge of recognition phenomena at the surface of polymer brushes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2008.12.016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Glucomannan polysaccharides derived from Pediococcus pentosaceus: From molecular characteristics to inhibition on colonic cancer cells proliferation.
Carbohydr Polym
November 2025
College of Food Science, Shihezi University, Shihezi 832003, China; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Key Laboratory of Traditional Chinese Dough Processing, Tianjin 300203, China; Gulbali Institute-Agriculture Water
Extracellular polysaccharides (EPS) from Pediococcus pentosaceus were obtained, followed by investigation of their structural and functional properties. Current results indicated the polysaccharides belonged to glucomannans, which mainly consisted of mannose and glucose with a molecular weight of 2248.71 kDa.
View Article and Find Full Text PDFMannose trimming is the dominant signal for the release of misfolded glycoproteins from ER quality control.
J Biol Chem
August 2025
Institute of Plant Biotechnology and Cell Biology, Department of Biotechnology and Food Sciences, BOKU University; Vienna, Austria. Electronic address:
N-glycosylation is essential for protein folding in the endoplasmic reticulum (ER). Glycan attachment facilitates the binding of newly synthesised polypeptides to calnexin and calreticulin, two ER-resident lectins that act as chaperones and promote folding. The regulatory mechanism underlying this process is dictated by the glycan composition, and this study has elucidated the function of mannose trimming in the release of misfolded glycoprotein from ER quality control and subsequent transfer to ER-associated degradation (ERAD) in plants.
View Article and Find Full Text PDFAntimicrobial and mosquito larvicidal properties of silver nanoparticle synthesized by extracellular polysaccharide from Psychrobacter okhotskensis.
Int J Biol Macromol
August 2025
Microbiology section, Department of Botany, The University of Burdwan, Golapbag, Purba Bardhaman 713104, West Bengal, India. Electronic address:
Rapid increase in multidrug-resistant (MDR) bacteria and mosquito-borne deadly diseases constitutes a significant global health issue, necessitating the development of novel therapeutics. This study focused on an effective approach performed to biosynthesis of silver nanoparticle (AgNPs) by using extracellular polysaccharide (EPS) first extracted from Psychrobacter okhotskensis PCRB17b. The EPS showed thermostable, low molecular weight, crystalline, and heteropolysaccharide which predominantly contain mannose (79.
View Article and Find Full Text PDFN-glycans in lung tissue specimens: a prospective target for enhanced cancer diagnosis and prognosis.
J Transl Med
August 2025
The Institute of Human Virology, University of Maryland, 725 W. Lombard St, Baltimore, MD, 21201, USA.
Background: It is well established that the cancerous transformation of cells is accompanied by profound alterations in glycosylation. In this study, we demonstrate the diagnostic potential of N-glycan profiling in tissue specimens from patients, primarily representing the two major types of lung cancer: non-small cell and small cell lung cancer.
Methods: Lung tissues and biopsies obtained from surgery and bronchoscopy underwent sample processing and enzymatic digestion.
Unraveling the characteristic structures of Cuscuta chinensis Lam. polysaccharides and its inhibitory effects on non-alcoholic fatty liver disease.
Int J Biol Macromol
September 2025
Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China.
Cuscuta chinensis Lam. is a well-known edible and medicinal plant in China, generally used in traditional medicine with meridian tropism into liver and kidney. In this work, two fractions (CCL-A and CCL-B) were isolated and purified from the polysaccharides of Cuscuta chinensis Lam.
View Article and Find Full Text PDF