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Article Abstract
We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC497642 | PMC |
| http://dx.doi.org/10.1128/JCM.42.8.3809-3812.2004 | DOI Listing |
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