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J Appl Microbiol
September 2025
Graduate Institute of Medical Sciences, National Defense Medical University, Taipei City 114201, Taiwan (R.O.C.).
Aims: This study aims to develop and evaluate a rapid and high-multiplex pathogen detection method for clinical and food specimens to address the ongoing public health threat of foodborne infections and the limitations of conventional culture-based diagnostics.
Methods And Results: The foodborne bacteria (FBB) assay integrates multiplex PCR, T7 exonuclease hydrolysis, and a suspension bead array to simultaneously detect 16 genes from 13 major foodborne bacteria. Analytical performance was evaluated using reference strains, while diagnostic performance was assessed using clinical and food samples.
Cell Mol Biol (Noisy-le-grand)
September 2025
Department of Biology, College of Education for Pure Sciences, University of Kerbala, Kerbala, Iraq.
Gastric cancer is one of the causes of deaths related to cancer across the globe and both genetic and environmental factors are the most prominent. Causes of its pathogenesis. This paper researches the expression of the C-FOS gene.
View Article and Find Full Text PDFJACC Cardiovasc Imaging
September 2025
Division of Cardiovascular Prevention and Wellness, Department of Cardiology, Houston Methodist DeBakey Heart and Vascular Center, Houston, Texas, USA.
Background: Coronary computed tomography angiography (CTA)-derived plaque burden is associated with the risk of cardiovascular events and is expected to be used in clinical practice. Understanding the normative values of computed tomography-based quantitative plaque volume in the general population is clinically important for determining patient management.
Objectives: This study aimed to investigate the distribution of plaque volume in the general population and to develop nomograms using MiHEART (Miami Heart Study) at Baptist Health South Florida, a large community-based cohort study.
Med Vet Entomol
September 2025
Centro de Bioinvestigaciones-CeBio, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires-CIT NOBA (CONICET-UNNOBA-UNSAdA), Pergamino, Argentina.
Fleas (Insecta: Siphonaptera) are recognised vectors of bacteria that affect human and other animal health, whose reservoirs are in the majority mammals. Among these, some species of the genera Rickettsia (Rickettsiales: Rickettsiaceae) and Bartonella (Rhizobiales: Bartonellaceae) are emerging and re-emerging throughout the world; however, their circulation across vast regions of Argentina and numerous animal species, particularly wild species remains largely unknown. The study of wild animal roadkill provides valuable insights into parasitic associations and the presence of pathogenic microorganisms, allowing the generation of a health alert in certain ecosystems.
View Article and Find Full Text PDFJ Virol
September 2025
Department of Microbiology, Immunology and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, Kentucky, USA.
Arthropod-borne viruses (arboviruses) pose a major threat to global public health, impacting both human and animal health. Genomic characterization is important for arboviruses because it allows for an understanding of their evolution and improves timely outbreak and epidemic response. In this study, we used high-throughput sequencing and computational analyses to characterize the genomes and evolution of 46 previously unsequenced or partially sequenced arbovirus isolates collected across 23 countries between 1954 and 1984.
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