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Purpose: To introduce a true three-dimensional (3D) tagging technique for the assessment of myocardial tissue motion.
Materials And Methods: To generate a 3D tagging grid, a complementary spatial modulation of magnetization (CSPAMM) was applied in three spatial directions. Imaging was performed using a conventional fast 3D gradient-echo sequence. For automatic analysis of the 3D-CSPAMM data set, evaluation software, based on a 3D extension of the HARP technique, was used.
Results: Successful application of the 3D-CSPAMM technique in healthy subjects allowed the accurate determination of quantitative 3D motion patterns in the human heart.
Conclusion: 3D-CSPAMM may contribute to the quantification of the local 3D myocardial motion pattern throughout the cardiac cycle.
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http://dx.doi.org/10.1002/jmri.10145 | DOI Listing |
J Vis Exp
July 2025
Department of Chemistry, University of Kentucky;
Understanding receptor assembly is critical for elucidating the mechanisms underlying their function and regulation in physiological processes. While traditional in vitro single-molecule studies rely on isolating proteins from heterologous expression systems, they often fail to capture the in vivo physiological complexity involved in the organization and assembly of cell surface proteins. This protocol employs Total Internal Reflection Fluorescence Microscopy (TIRFM) to study GFP-tagged Ryanodine Receptor 2 (RyR2) molecules encapsulated within nanoscale vesicles.
View Article and Find Full Text PDFJ Gen Physiol
September 2025
School of Natural Sciences, University of Kent, Canterbury, UK.
β-cardiac myosin mediates cardiac muscle contraction within the sarcomere by binding to the thin filament in an ATP-powered reaction. This process is highly regulated on a beat-to-beat basis by calcium interactions with the thin filament, but also contractile force is highly regulated by controlling the number of myosins available, resulting in a dynamic reserve. Our goal was to examine the size of this reserve and how it is modulated by cardiac myosin binding protein-C (cMyBP-C).
View Article and Find Full Text PDFJ Cell Physiol
June 2025
Department of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.
Exercise and diet are the best-known methods for attenuating aging-related health decline. However, exercise in older age has diminished gains of strength and agility, and a danger of unrepaired muscle damage. Improving the understanding of age-related differences in response to exercise, our results demonstrate that in old mice, downhill treadmill (eccentric) exercise causes increased influx of CD45+ cells (inflammation) and fibrotic index (fibrosis) in the heart and skeletal muscles.
View Article and Find Full Text PDFCirculation
July 2025
Beijing Key Laboratory of Cardiovascular Receptors Research, China (J.Z., Y.Z.).
Background: Myocardial ischemia/reperfusion (I/R) injury is a substantial challenge to the management of ischemic heart disease, the leading cause of mortality worldwide. Arachidonic acid (AA) is a prominent polyunsaturated fatty acid in the human body and plays an important role in various physiological and pathological conditions. AA metabolic enzymes determine AA levels; however, currently there is no comprehensive analysis of AA enzymes in cardiac I/R injury.
View Article and Find Full Text PDFJ Vis Exp
March 2025
Department of Physiology and Biophysics, University of Mississippi Medical Center;
Metabolic reprogramming is a hallmark of monocyte/macrophage activation and polarization between pro- and anti-inflammatory states. For example, pro-inflammatory (i.e.
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