Exploring the characters of sperm lipid peroxidation, enzymatic antioxidant activity, and spermatozoa zona binding capacity with aging in dogs.

This study aimed to assess differences in fresh semen quality between mature and senile dogs, focusing on oxidative stress markers, including spermatozoa lipid peroxidation and enzymatic antioxidant activity. Furthermore, the ability of frozen-thawed spermatozoa from these age groups to bind to the zona pellucida was investigated. Forty clinically healthy dogs were categorized into two groups: mature (3 ± 0.

Association between sperm DNA fragmentation and fertility parameters in farm animals: a systematic review and meta-analysis.

Background: Accurately predicting male fertility is crucial for the animal breeding industry due to its significant economic implications. Existing literature suggests that mammalian fertility is partially dependent on sperm DNA integrity. However, routine semen analysis often fails to detect DNA damage and does not consistently correlate with field fertility outcomes.

Analyzing estrous cycle dynamics with different protocols of 4.7 mg deslorelin implant aplication in bitches.

The use of deslorelin acetate implants for estrus induction is increasingly popular, but optimal timing for administration and removal remains uncertain. This study aimed to compare the effectiveness of estrus induction during early (90-120 days after heat cessation, G1: n = 7) and late anestrus (≥160 days after heat cessation, G2: n = 7; G3: n = 7) and at different implant removal times (Progesterone (P4) ≥ 3 ng/ml (G1 = 3.5 ± 0.

Deciphering the mechanism of sperm cell aging - Functional, structural, and kinematic characteristics in fresh semen of mature and aged dogs.

This study aimed to evaluate fresh semen from mature and senile dogs, identify differences in sperm parameters, and determine the correlations between sperm variables and semen quality. Forty dogs were divided into two groups based on age: mature, aged 2-4 years (n = 20); and senile, aged ≥ 9 years (n = 20). Two or three ejaculates, totaling 107 samples, were collected from each dog and evaluated for various parameters including motility and sperm movement kinematics, morphology and viability, membrane lipid disorder, DNA integrity, membrane integrity, mitochondrial potential, seminal plasma total antioxidant capacity, apoptotic-like changes, and proAKAP4 protein levels.