Heterologous N- and C-terminal domains of 3D-cry proteins form a functional operon enabling natural crystallization and nematicidal activity.

Bacillus thuringiensis (Bt) is an insect pathogen that primarily relies on pore-forming toxins known as Cry proteins to kill its insect larval hosts. The effectiveness of Cry proteins has driven a worldwide search for Bt strains to identify and characterize novel insecticidal proteins with different specificities. In this study, Bt genome analysis revealed two consecutive open reading frames that are highly similar to the N-terminal of Cry14Aa1 and the C-terminal of Cry21Ca2, both of which target nematodes.

Characterization and Genome Analysis of a Novel Mu-like Phage VW-6B Isolated from the Napahai Plateau Wetland of China.

Although bacteriophages are more numerous and have smaller genomes than their bacterial hosts, relatively few have their genomes sequenced. Here, we isolated the Pseudomonas fluorescens bacteriophage from Napahai plateau wetland and performed de novo genome sequencing. Based on the previous biological characteristics and bioinformatics analysis, it was determined that VW-6B was a linear double-stranded DNA (dsDNA) phage with 35,306 bp, with 56.

The strong inhibitory effect of combining anti-cancer drugs AT406 and rocaglamide with blue LED irradiation on colorectal cancer cells.

There is still no satisfying method to treat colorectal cancer (CRC) currently. Inspired by cocktail therapy, the combination of 465 nm blue LED irradiation and two multi-target anticancer agents AT406 and Rocaglamide has been investigated as an innovative way to treat colorectal cancer cells in vitro. It showed a strong inhibitory effect on colorectal cancer cells, and its side effects on human normal cells are negligible.