Exploring the influence of sex and age on the quality and flavor of Huai goat longissimus thoracis et lumborum through transcriptomic and metabolomic analyses.

Background: The aim of this study was to investigate the effects of age and sex on the composition of fatty acids, amino acids, and vitamins in the longissimus thoracis et lumborum (LTL) of Huai goats.

Methods: The longissimus thoracis et lumborum tissues of eight Huai goats were collected postslaughter and analyzed for amino acids, fatty acids, and vitamins by gas chromatography‒mass spectrometry (GC‒MS) and standard methods. RNA sequencing (RNA-Seq) was conducted to identify differentially expressed genes (DEGs) across different ages and sexes.

Sulfation, anticoagulant and antioxidant activities of polysaccharide from green algae Enteromorpha linza.

Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation, molecular weight, substitution position and chain conformation. In present study, the polysaccharide with low molecular weight (LEP) from Enteromorpha linza was sulfated with chlorosulfuric acid in formamide. The obtained polysaccharide sulfate was selected to evaluate their antioxidant activities and the anticoagulant activity in the coagulation assays, activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT).