NIRS-based fresh grape ripeness prediction with SPA-LASSO spectral feature selection.

A rapid and non-destructive maturity evaluation model based on Near-Infrared Spectroscopy (NIRS) is proposed for monitoring quality parameter changes during the ripening process of fresh grapes and determining the optimal harvest period. Initially, physicochemical parameter variations of Cabernet Sauvignon grapes across twelve growth stages were studied to support predictions. Subsequently, SPA-LASSO was used to select feature wavelengths from five preprocessed full spectra, and Partial Least Squares Regression (PLSR) was employed to establish models predicting Soluble Solid Content (SSC) and Total Acid (TA) levels.

A novel attenuated and marker -BoAHV-1 combined vaccine provides broad protection against diverse bovine alphaherpesvirus type 1 genotypes.

() and bovine alphaherpesvirus 1 (BoAHV-1) are two major infections that seriously impair cattle's reproductive and respiratory systems. We created an -BoAHV-1 combined vaccine and assessed its safety and effectiveness in our earlier work. This study evaluated a novel combined vaccine comprising attenuated and marker BoAHV-1 antigens for its immunogenicity and efficacy in protecting cattle against diverse BoAHV-1 genotypes.

A Genome-Wide Screening of Novel Immunogenic TrLSDV103 Protein of Lumpy Skin Disease Virus and Its Application for DIVA.

Lumpy skin disease (LSD) is an infectious cattle disease caused by the lumpy skin disease virus (LSDV), posing a serious threat to the livestock industry. This study aimed to identify specific immunogenic targets for differential diagnosis and potential vaccine development. Using a phage display library covering the entire LSDV proteome, we screened sera from naturally LSDV-infected cattle and those vaccinated with the live attenuated goatpox virus vaccine (GTPV AV41) to identify differential antibody-binding viral peptides, leading to the identification of peptides within the LSDV103 protein.

The Establishment of a Novel γ-Interferon In Vitro Release Assay for the Differentiation of -Infected and BCG-Vaccinated Cattle.

BCG vaccination is increasingly reconsidered in the effective prevention of bovine tuberculosis (bTB). However, the primary challenge in BCG vaccination for cattle is the lack of a technique for differentiating between infected and vaccinated animals (DIVA). This study aimed to establish a novel DIVA diagnostic test based on an interferon-gamma in vitro release assay (IGRA).

Prevalence, Molecular Characteristics and Virulence Identification of Bovine Parainfluenza Virus Type 3 in China.

Bovine parainfluenza virus type 3 (BPIV-3) is one of the major pathogens of the bovine respiratory disease complex (BRDC). BPIV-3 surveillance in China has been quite limited. In this study, we used PCR to test 302 cattle in China, and found that the positive rate was 4.

Predicting Protein-Protein Interactions via Gated Graph Attention Signed Network.

Protein-protein interactions (PPIs) play a key role in signal transduction and pharmacogenomics, and hence, accurate PPI prediction is crucial. Graph structures have received increasing attention owing to their outstanding performance in machine learning. In practice, PPIs can be expressed as a signed network (i.