Designing a multiepitope mRNA-based vaccine against enterotoxigenic infection in calves: Immunoinformatics and molecular modeling approach.

Background: is one of the serious pathogens causing various infections in the animal field, such as neonatal calf diarrhea, which is responsible for mortality associated with diarrhea during the first days of life.

Aim: Current work is aimed at designing an effective and safe multiepitope vaccine candidate against infection in calves based on the fimbrial protein K99 of Enterotoxigenic (ETEC) and Immuno-informatics.

Methods: A conserved sequence of K99 protein was generated, and then highly antigenic, nonallergic, and overlapped epitopes were used to construct a multiepitope vaccine.