P300 Promotes RUNX1 Transcription to Regulate Autophagy and Drive Corneal Neovascularization.

Purpose: This study aimed to elucidate the regulatory role of Runt-related transcription factor 1 (RUNX1) in corneal neovascularization (CoNV) and its possible mechanisms.

Methods: In VEGF-induced human umbilical vein endothelial cells (HUVECs), lentiviral vectors were used to knock down or overexpress RUNX1, and plasmid transfection was employed to knock down P300. EdU assay, Transwell assay, and tube formation assay were conducted to assess cell proliferation, migration, and tube formation ability, respectively.

Global, regional and national burden of Metabolic dysfunction-associated steatotic liver disease in adolescents and adults aged 15-49 years from 1990 to 2021: results from the 2021 Global Burden of Disease study.

Background: With rising obesity and diabetes rates, the incidence of Metabolic dysfunction-associated steatotic liver disease (MASLD) among individuals aged 15-49 is increasing, affecting their productivity, health, and quality of life. However, a comprehensive global assessment of MASLD burden and long-term trends in this age group remains lacking.

Methods: The study analyzed prevalence, incidence, mortality, and disability-adjusted life years (DALYs) for individuals aged 15-49 using data from the Global Burden of Disease (GBD) 2021 study.

Exploring the Efficacy of AZD6738 in Corneal Neovascularization: Autophagy Enhancement and Angiogenesis Inhibition.

This study aimed to investigate the therapeutic potential of AZD6738, an ataxia-telangiectasia and rad3-related (ATR) kinase inhibitor, in preventing corneal neovascularization (CNV) by exploring its effects on autophagy regulation and angiogenesis. Human umbilical vein endothelial cells were cultured and treated with varying concentrations of AZD6738 and vascular endothelial growth factor (VEGF) to assess cell viability, migration, and tube formation. A corneal alkali burn model in Sprague-Dawley rats was established to evaluate the effects of AZD6738 on CNV.