Synergistic improvement of biohydrogen yield from food wastewater using Clostridium puniceum and magnetite-biochar.

Biohydrogen production using waste resources and microorganisms was gaining attention as a clean energy alternative that could reduce production cost and environmental pollution. Among waste resources, food wastewater (FW) was especially suitable due to its high lactate content, which supported microbial hydrogen production. However, FW also contained inhibitory compounds that could limit microbial activity.

Retainment of Poly(3-hydroxybutyrate--3-hydroxyhexanoate) Properties from Oil-Fermented Using Additional Ethanol-Based Defatting Process.

Engineering of could enable the production of various polyhydroxyalkanoates (PHAs); particularly, poly(3-hydroxybutyrate--3-hydroxyhexanoate) (P(3HB--3HH)), a biopolymer with enhanced mechanical and thermal properties compared to poly(3-hydroxybutyrate) (PHB), can be efficiently produced from vegetable oils. However, challenges remain in the recovery process, particularly in removing residual oil and minimizing degradation of the polymer structure during extraction steps. This study investigated the effects of ethanol-based defatting on the recovery and polymeric properties of P(3HB--3HH).

Development of a DNA endonuclease I-SceI-based scarless genome editing system for Cupriavidus necator.

Cupriavidus necator is a promising microbial chassis capable of fixing CO₂ and producing high polyhydroxyalkanoate yields. Consequently, various genetic engineering methods have been explored. While sacB-based homologous recombination (HR) and CRISPR-Cas9 have shown both advantages and disadvantages in C.

Discovery of a High 3-Hydroxyhexanoate Containing Poly-3-hydroxybutyrate--3-hydroxyhexanoate Producer-, sp. Oh_1 with Enhanced Fatty Acid Metabolism.

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB--3HHx)) is a representative PHA copolymer that can improve the mechanical limitations of polyhydroxybutyrate (P(3HB)). Although genetic engineering can facilitate 3HHx incorporation, it often compromises cell growth and reduces polymer molecular weight owing to metabolic disruptions caused by the deletion of acetoacetyl coenzyme A (acetyl-CoA) reductase (PhaB). To address this issue, native strains capable of producing high levels of 3HHx were identified via oil-based screening.

Sucrose-Based Screening of a Novel Strain, sp. YI8, and Its Application to Polyhydroxybutyrate Production from Molasses.

Poly(3-hydroxybutyrate) is a biodegradable plastic produced by various microbes. Considering the emerging environmental problems caused by plastics, P(3HB) has gained attention as a substitute for conventional plastics. In this study, we isolated a novel P(3HB)-producing microbe, sp.

Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440.

Levulinic acid (LA) is a platform compound regarded as a promising organic intermediate for the synthesis of various chemicals such as fuel additives, plasticizers, solvents, and pharmaceuticals. Traditionally, LA is produced via acid-catalyzed dehydration and hydrolysis of lignocellulosic biomass, but this process involves challenges such as high temperatures and pressures, the use of strong acids, byproducts formation, and limitations in recovery and purification. To provide an alternative for chemical synthesis, we previously designed an integrated process to produce LA from glucose using genetically engineered Pseudomonas putida KT2440.

Effect of SAM-Dependent Methyltransferases from sp. YLGW01 on Phospholipid Fatty Acids Composition and Production of Polyhydroxalkanoates in .

The bacterial membrane changes in response to growth conditions such as salt concentration, temperature, and growth inhibitors. As membrane fatty acid related genes are important in controlling the membrane composition, we studied two genes cyclopropane fatty acid synthase () and cis-vaccinate 11-methyltransferase () that designated to S-adenosylmethionine (SAM)-dependent methyltransferase derivatives from sp. YLGW01.

Real-time monitoring method of microbial growth using a simple pressure-based respiration detection system.

Dry cell weight (DCW) and optical density (OD) measurement methods provide useful data for assessing microbial growth. However, their sampling process is labor-intensive and time-consuming. Therefore, we aimed to evaluate a method for measuring microbial growth through continuous CO measurement under aerobic conditions using a pressure-based respiration detection system, which is traditionally used in anaerobic environments and applies measurement of reduced pressure by capturing CO with KOH.

Enhanced production of extracellular triacylglycerol lipase for bioplastic degradation by replacing signal peptide.

With the increase in plastic production, efficient and timely plastic degradation are urgently needed. In that point, biodegradable plastics have attracted attention as potential solutions for environmental pollution of plastics. However, finding of superior degrading strains and enzymes such as esterase, cutinase, and triacylglycerol lipase (TGL) of bioplastic are still needed together with the efficient secretion systems of degrading enzymes.

An all-in-one strategy for the simultaneous production of bioplastics and degrading enzymes in engineered Escherichia coli.

Bioplastics are promising alternatives for traditional plastics, which contribute significantly to environmental pollution and have a detrimental impact on ecosystems. To advance their use, further research into bioplastic biodegradation is essential. In this study, we propose a novel approach for simultaneous polyhydroxybutyrate (PHB) and degrading enzyme production in a single-cell system using engineered Escherichia coli.

High-Yield Production of Polyhydroxybutyrate and Poly(3-hydroxybutyrate--3-hydroxyhexanoate) from Crude Glycerol by a Newly Isolated Species Oh_219.

Crude glycerol (CG), a major biodiesel production by-product, is the focus of ongoing research to convert it into polyhydroxyalkanoate (PHA). However, few bacterial strains are capable of efficiently achieving this conversion. Here, 10 PHA-producing strains were isolated from various media.

Development of Organic/Inorganic Hybrid Materials for Fully Degradable Reactive Oxygen Species-Releasing Stents for Antirestenosis.

Despite innovative advances in stent technology, restenosis remains a crucial issue for the clinical implantation of stents. Reactive oxygen species (ROS) are known to potentially accelerate re-endothelialization and lower the risk of restenosis by selectively controlling endothelial cells and smooth muscle cells. Recently, several studies have been conducted to develop biodegradable polymeric stents.