Publications by authors named "Yannick Flaskamp"
Background: Cell-free protein synthesis (CFPS) is one approach to address the increasing demand for complex recombinant proteins in various applications, especially in the pharmaceutical sector. CFPS offers a variety of advantages like the ability to express cytotoxic proteins, no need for transformations or screening of strains and, thus, reduced production times. Often industrially relevant proteins require post-translational modifications (PTM).
View Article and Find Full Text PDFEukaryotic cell-free protein synthesis (CFPS) can accelerate expression and high-throughput analysis of complex proteins with functionally relevant post-translational modifications (PTMs). However, low yields and difficulties scaling such systems have prevented their widespread adoption in protein research and manufacturing. Here, we provide detailed demonstrations for the capabilities of a CFPS system derived from Nicotiana tabacum BY-2 cell culture (BY-2 lysate; BYL).
View Article and Find Full Text PDFArticle Synopsis
- - Eukaryotic cell-free protein synthesis (CFPS) systems offer a faster and simplified method for expressing complex proteins with important post-translational modifications, but challenges like low yields and scalability have limited their use.
- - The BY-2 cell culture-derived CFPS system (BYL) can produce functional proteins in high yields (up to 3 mg/mL) within 48 hours, achieving native modifications like disulfide bonds and N-glycosylation, with a commercial version called 'ALiCE.'
- - Researchers have successfully scaled the BYL CFPS process from small volumes (100 μL) to larger batches (10 mL, 100 mL, and even 1 L)