['Hyperglycaemic memory' in human umbilical vein endothelial cells mediated by interacting loop of protein kinase Cbeta(2)-reactive oxygen species].

Objective: To investigate the roles of protein kinase C (PKC) beta(2) and reactive oxygen species (ROS) in the mRNA expression of vascular endothelial growth factor(VEGF) and vascular cell adhesion molecule-1(VCAM-1) in human umbilical vein endothelial cells (HUVECs), imitating 'hyperglycemic memory' in vitro and the relationship between them.

Methods: The HUVECs were divided into 5 groups: normal glucose (NG, D-glucose 5 mmol/L x 3 weeks) group, high glucose (HG, D-glucose 25 mmol/L x 3 weeks) group, memory (M, 25 mmol/L D-glucose x 2 weeks + 5 mmol/L D-glucose x 1 weeks) group, memory transfected with PKCbeta(2) (MB, M + Ad5-PKCbeta(2)) group, memory plus alpha-lipoic acid (MA, M + 62.5 micromol/L alpha-lipoic acid) group.

[To compare and research the clinical effect of treating the comminute distal radius fractures by refining splintage].

Objective: To treat the comminute distal radius fractures by improved splint, and obersve the curative effect and compare the radiology with the traditional splint.

Methods: Ninety-two patients with distal radius comminute fracture were randomly divided into two groups. There were 38 males and 54 females.