Free Fatty Acids Promote the Development of Prostate Cancer by Upregulating Peroxisome Proliferator-Activated Receptor Gamma.

Introduction: As one of the most common forms of cancer that threatens men's health, prostate cancer (PCa) is under a trend of increasing morbidity and mortality in most countries. More and more studies have pointed out that obesity is closely linked to the occurrence and development of PCa, although there are still many undiscovered molecular mechanisms between the two.

Methods: In the present study, we compare serum lipid levels in patients with PCa and normal individuals.

The Effect and Mechanism of TLR9/KLF4 in FFA-Induced Adipocyte Inflammation.

Objective: Current research has reported that obesity is a chronic inflammatory state, which is closely related with excessive accumulation of free fatty acid, while the specific mechanism that high level of FFA causes inflammation is not very clear. Thus, our research intended to observe the high FFA effects on TLR9/KLF4 expression and the downstream inflammatory factors, to explore the mechanism of inflammatory response suppressed by TLR9/KLF4.

Methods: qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of TLR9, KLF4, and key inflammation-related factors.

The Effect and Mechanism of KLF7 in the TLR4/NF-B/IL-6 Inflammatory Signal Pathway of Adipocytes.

Objective: To investigate the role and possible molecular mechanism of Krüppel-like factor 7 (KLF7) in the TLR4/NF-B/IL-6 inflammatory signaling pathway activated by free fatty acids (FFA).

Methods: The mRNA and protein expression levels of KLF7 and the factors of TLR4/NF-B/IL-6 inflammatory signal pathways were detected by qRT-PCR and Western blotting after cell culture with different concentrations of palmitic acid (PA). The expression of KLF7 or TLR4 in adipocytes was upregulated or downregulated; after that, the mRNA and protein expression levels of these key factors were detected.

Comparative gene expression profile and DNA methylation status in diabetic patients of Kazak and Han people.

We attempted to investigate the mechanism and susceptibility genes for diabetes in Han and Kazak ethnic individuals.The abdominal omental adipose tissues were obtained from diabetic cases and healthy controls. The gene expression and methylation data were produced for Kazak and Han individuals, respectively, and analyzed by bioinformatics analysis.

The effects of fat-induced obesity on bone metabolism in rats.

Objective: The aim of the current study was to investigate the effects of obesity, induced via a high-fat diet, on bone metabolism in rats.

Methods: Two hundred healthy Wistar male rats aged 4 weeks were fed a standard diet and a high-fat diet. At specific time points (week 0, 4, 6, 8, and 10), plasma was collected to determine the levels of glucose and lipid metabolism.

Correlation of TLR4 and KLF7 in Inflammation Induced by Obesity.

Objective Recent studies have revealed a link between toll-like receptors (TLRs), Kruppel-like factors (KLFs), and the adipose tissue inflammation associated with obesity. TLR4 is associated with chronic inflammation in obesity. KLF7 is known to play an important role in the differentiation of adipocytes, but its role in visceral adipose tissue inflammation has not yet been investigated.

DNA methylation of tumor necrosis factor-α, monocyte chemoattractant protein-1, and adiponectin genes in visceral adipose tissue is related to type 2 diabetes in the Xinjiang Uygur population.

Background: The higher probability of type 2 diabetes mellitus (T2DM) in the Uygur population is due to a greater waist:  hip ratio and visceral fat. This study investigated DNA methylation of tumor necrosis factor-α (TNF), monocyte chemoattractant protein-1 (MCP1), and adiponectin (ADIPOQ) in visceral adipose tissue in T2DM.

Methods: Visceral adipose tissue was collected from Uygur individuals and divided into normal control (NC; n = 50), obese (Ob; n = 48), and T2DM (n = 26) groups.

Correlation of A2bAR and KLF4/KLF15 with Obesity-Dyslipidemia Induced Inflammation in Uygur Population.

In this paper, the researchers collected visceral adipose tissue from the Uygur population, which were divided into two groups: the normal control group (NC, n = 50, 18.0 kg/m(2) ≤ BMI ≤ 23.9 kg/m(2)) and the obese group (OB, n = 45, BMI ≥ 28 kg/m(2)), and then use real-time PCR to detect the mRNA expression level of key genes involved in inflammation signaling pathway.