Optimizing Rooster Semen Preservation: Effect of Oxygen Exposure, Sample Rotation, and HEPES Buffer Supplementation.

This study aimed to investigate physical and biochemical strategies to optimize the preservation and fertilizing capacity of rooster semen during chilled storage and after artificial insemination (AI), respectively. Two semen extenders-0.9% sodium chloride (NaCl) and IGGKPh-were evaluated through two factorial experiments.

Integrating Genomic Selection and Genome-Wide Association Study to Enhance Reproductive Traits in Thai Swamp Buffalo.

Reproductive inefficiencies, such as delayed age at first calving (AFC) and a prolonged calving interval (CI), hinder the productivity of Thai swamp buffaloes. This study aimed to improve the genetic evaluation of these traits by integrating genomic selection (GS) and genome-wide association studies (GWASs). Reproductive records (n = 1034) and genotypes (n = 474) from swamp buffaloes across Thailand were analyzed.

Genetic Evaluation of Growth Traits in Black-Boned and Thai Native Synthetic Chickens Under Heat Stress.

Heat stress is a critical constraint to poultry production in tropical regions, where the temperature-humidity index (THI) frequently exceeds thermoneutral thresholds. Despite growing interest in climate-resilient livestock, limited research has explored the genetic sensitivity of local chicken breeds to increasing THI levels. This study aimed to evaluate the genetic effects of increasing THI on growth performance traits in two tropical chicken breeds.

Long-Term Heat Stress and Genetic Responses in Growth Traits of Thai Native Synthetic Chicken Lines.

In this study, we evaluated genetic parameters and progress in growth traits and heat tolerance across four Thai native synthetic chicken lines-Kaimook e-san, Soi Pet, Soi Nin, and Kaen Thong-over seven generations. Growth traits, including body weight (BW), average daily gain (ADG), and breast circumference (BrC), were recorded and analyzed from hatching to 14 weeks of age, with heat stress assessed based on the temperature-humidity index (THI). Genetic parameters were estimated using a multi-trait animal model incorporating THI thresholds.

Genetic perspectives on thermal tolerance in native chickens using non-linear growth models and temperature-humidity indices.

The aim of this study was to determine the best non-linear model, between the Gompertz and sinusoidal models, for estimating the absolute growth rate (AGR) of Pradu Hang Dum chickens and examine the appropriate temperature-humidity index (THI) equation for studying heat stress in these chickens. The growth of Pradu Hang Dum chickens was monitored from hatching to 16 weeks of age. Males consistently had higher body weights than females, with the difference becoming more pronounced over time.

A novel temperature-humidity index-based model for evaluating semen characteristics in Thai native roosters under tropical conditions.

Temperature and relative humidity indices (THIs) are widely used to study the effects of heat stress on reduced poultry production traits. However, a suitable THI for reproductive traits has not yet been established. This study aimed to develop a novel THI to predict semen traits in native roosters using regression analysis.

Improvement of Sperm Quality in Cryopreserved Boar Semen by Supplementation of Kaempferia parviflora and Cholesterol-Loaded Cyclodextrin in Semen Extender.

We investigated the potential of Kaempferia parviflora (KP) extract, rich in phenolic compounds, for enhancing boar semen quality during cryopreservation. We evaluated the impact of various KP extract concentrations (0%-8% w/v) in freezing extenders on postthawed semen quality and oxidative stress markers, along with the effects of cholesterol-loaded cyclodextrins (CLC). Semen was frozen using liquid nitrogen vapor before being plunged into liquid nitrogen, and postthaw semen quality and lipid peroxidation were analyzed.

The Role of Black Soldier Fly Larvae in Optimizing Performance and Amino Acid Composition in Thai Native Chicken.

The objective of this study was to investigate the effects of black soldier fly larvae (BSFL) inclusion on the performance, economic return, carcass traits, meat quality, amino acid profile, and purine content in the breast meat of Thai native chickens (TNCs) (Pradu Hang Dam Mor Kor 55: PD) from 10 to 70 days of age. A total of were randomly assigned to three dietary treatments with four replicates per treatment (18 birds per replicate, with a 1:1 male-to-female ratio). The treatments included (T1) control diet with soybean meal as the main protein source, (T2) 10% BSFL meal replacing soybean meal, and (T3) 12% BSFL meal replacing soybean meal.

Pleiotropic Genes Affecting Milk Production, Fertility, and Health in Thai-Holstein Crossbred Dairy Cattle: A GWAS Approach.

Understanding the genetic basis of economically important traits is essential for enhancing the productivity, fertility, and health of dairy cattle. This study aimed to identify the pleiotropic genes associated with the 305-day milk yield (MY305), days open (DO), and milk fat-to-protein ratio (FPR) in Thai-Holstein crossbred dairy cattle using a genome-wide association study (GWAS) approach. The dataset included 18,843 records of MY305 and milk FPR, as well as 48,274 records of DO, collected from first-lactation Thai-Holstein crossbred dairy cattle.

Genetic Strategies for Enhancing Rooster Fertility in Tropical and Humid Climates: Challenges and Opportunities.

Native chickens are important both economically and for the preservation of genetic diversity, especially for optimizing the reproductive performance of male chickens, which plays an important role in genetic propagation. However, hot and humid weather affects the quality of semen and the breeding ability of male chickens. One method used to solve this problem is genetic selection using genomic technology, which improves accuracy and shortens the breed selection time.

Pharmacokinetics and stability of methoxyflavones from in Thai native roosters.

Introduction: The present study aimed to characterize the pharmacokinetics and stability of methoxyflavones derived from (KP) in Thai native roosters after oral administration of a KP ethanolic extract.

Method: Twenty-seven male roosters were randomly divided into three groups and received KP extract at different doses of 100, 150, and 200 mg/kg of body weight. Plasma samples were prepared using acetonitrile, ethyl acetate, and a mixture of both to compare the optimal extraction efficiency.

Impact of heat stress on phenotypic and genetic parameters of egg fertility traits in four Thai native synthetic chicken lines.

Heat stress affects chickens as well as their eggs. Therefore, the objective of this study was to identify the temperature threshold at which heat stress began to negatively affect fertility traits and to estimate the genetic parameters of egg fertility (FER) in Thai native synthetic chicken lines under different thermal conditions. A total of 392,995 egg records from 5,919 individuals of four Thai synthetic chicken lines-Soi Nin, Soi Pet, Kaen Thong, and Kaimook e-san-were used in this study.

Effect of Supplementation in Semen Extenders on Post-Thaw Sperm Quality in Thai Native Bulls.

Cryopreservation is a widely used technique for long-term semen storage; however, it can induce oxidative stress, compromising sperm quality. This study examines the effect of (KP) supplementation in semen extenders on the post-thaw sperm quality of Thai native bulls. Fresh semen was collected and evaluated for motility, viability, and concentration prior to cryopreservation.

Optimization of rooster semen preservation: a comparative study of extender types and antioxidant supplementation strategies during cold storage.

This study evaluated the effect of semen extenders and antioxidant supplementation strategies on the quality and fertility of Thai native rooster semen during cold storage. In Experiment 1, four extenders (EK, IGGKPh, NaCl, and NCAB) were evaluated for their ability to preserve sperm motility, viability, lipid peroxidation (MDA), and oxidative stress (reactive oxygen species; ROS) over 24 h at 5°C. EK and IGGKPh extenders yielded superior motility (68.

Genetic strategies for enhancing litter size and birth weight uniformity in piglets.

This study aimed to estimate the genetic parameters and develop selection indices for litter size and birth weight uniformity in piglets. These traits are crucial for improving productivity and profitability of swine production. Data were collected from 9,969 litters of 4,465 sows and 106,463 piglets of various breeds from a farm in Thailand.

Determining the priority semen characteristics and appropriate age for genetic improvement in Thai native roosters.

Semen characteristics are crucial indicators of reproductive success and directly influence the production efficiency of native chickens, which play a vital role in sustainable poultry production systems in Thailand. Key semen traits, including mass movement, semen pH, semen volume, sperm viability, sperm abnormalities, and sperm concentration, are routinely evaluated to assess the reproductive performance of Thai native grandparent roosters. Improved reproductive efficiency in these chickens can lead to increased fertility rates, better hatchability, and enhanced productivity, significantly benefiting smallholder farmers and the poultry industry.

Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers.

This study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total of 117 chickens, including three Thai native breeds and commercial laying hens, were analyzed.

Research note: Improving post-thaw rooster sperm quality with Eurycoma longifolia extract and cyclodextrin.

Cryopreservation of rooster semen is essential for genetic conservation and breeding programs; however, the current methods result in a significant loss of viability. This study investigated the potential of Eurycoma longifolia (EL) extract and its cyclodextrin complex (EL-loaded cyclodextrin [ELC]) to improve rooster sperm cryopreservation. Supplementation with EL extract at 4 mg/mL significantly increased post-thaw total motility (62.

Enhancement of Semen Cryopreservation from Native Thai Bulls Through Leaf Extract Supplementation.

Cryopreservation of native Thai bull semen often results in significant post-thaw quality reduction, underscoring the need for effective cryoprotective strategies. This study investigated the effect of leaf extract (MOLE) as an antioxidant supplementation by incorporating four MOLE concentrations (0-1.5% [/]) into a standard semen extender, followed by cryopreservation using liquid nitrogen vapor freezing.

Integrating Genomic Selection and a Genome-Wide Association Study to Improve Days Open in Thai Dairy Holstein Cattle: A Comprehensive Genetic Analysis.

Days open (DO) is a critical economic and reproductive trait that is commonly employed in genetic selection. Making improvements using conventional genetic techniques is exceedingly challenging. Therefore, new techniques are required to improve the accuracy of genetic selection using genomic data.

Coenzyme Q10 Supplementation Effects on In Vitro Oocyte Maturation, Lipid Peroxidation, and Embryonic Development in Prepubertal and Aging Thai-Holstein Cows.

This study investigated the effects of coenzyme Q10 (CoQ10) supplementation on in vitro oocyte maturation, lipid peroxidation, and embryonic development in prepubertal and aging Thai-Holstein cows. First, we used slaughterhouse-derived oocytes to confirm that CoQ10 (50 μM) significantly enhanced cleavage (53.33% vs.

Genetic Profiles of Purine, Uric Acid, Superoxide Dismutase, and Growth in Thai Slow-Growing Chickens.

The objective of this study was to estimate genetic parameters and genetic correlations between growth characteristics and purine and uric acid in the breast and liver and superoxide dismutase (SOD) in the blood. The growth characteristics included body weight (BW) at hatching (BW0), BW at 2, 4, 6, 8, and 10 weeks of age, average daily gain (ADG) at 0-2, 2-4, 4-6, 6-8, and 8-10 weeks of age, and breast circumference at 6, 8, and 10 weeks of age (BrC6, BrC8, and BrC10) were recorded from 300 Thai native chickens (Shee breed). In total, 30 chickens (15 males and 15 females) were randomly euthanized to collect breast meat, liver, and blood samples to determine the purine content.

Effects of Black Soldier Fly Larvae Oil on Growth Performance, Blood Biochemical Parameters, Carcass Quality, and Metabolomics Profile of Breast Muscle of Thai Native Chickens.

This study aimed to evaluate the effects of the replacement of rice bran oil (RBO) with black soldier fly larvae oil (BSFLO) on growth performance, blood biochemicals, carcass quality, and metabolomics profile of breast muscle of Thai native chickens. A total of 192 1-day-old, mixed-sex, Pradu Hang Dam (Mor Kor 55) chickens were randomly allocated to one of three dietary groups. Each treatment had four replicates with 16 chicks per replicate (8 males and 8 females).

Impact of Heat Stress on Milk Yield, Milk Fat-to-Protein Ratio, and Conception Rate in Thai-Holstein Dairy Cattle: A Phenotypic and Genetic Perspective.

Heat stress severely affects dairy cattle production and reproduction performances in tropical regions. Genetic selection to maintain adequate yield and reproductive performance while enhancing their ability to withstand heat is essential for improving the genetics of dairy cows. Therefore, in this study, we aimed to estimate genetic parameters affecting production and reproduction performances under heat stress conditions in dairy cattle and to investigate the threshold point of heat stress for milk yield (MY), milk fat-to-protein ratio (FPR), and conception rate (CR) in Thai-Holstein dairy cattle.