Coupling proteomics and lipidomics for insights into regulation of oat (Avena sativa) grain lipid synthesis.

Breeding is a feasible strategy to develop low-oil oat (Avena sativa) varieties, which aligns with specific processing needs and dietary preferences. To identify factors contributing to a low-oil phenotype, we optimised a sequential extraction workflow for proteomics and lipidomics analyses on five Australian oat varieties with different oil contents. Oat oil content positively correlated with abundances of several proteins in lipid synthesis pathways, suggesting their key lipid regulatory roles.

Sodium doping and trapped ion mobility spectrometry improve lipid detection for novel MALDI-MSI analysis of oats.

Oat (Avena sativa L.) is an important cereal grain with a unique nutritional profile including a high proportion of lipids. Understanding lipid composition and distribution in oats is valuable for plant, food and nutritional research, and can be achieved using MALDI mass spectrometry imaging (MALDI-MSI).