Media formulation using statistical methodology to enhance α-amylase production for green synthesis of Au-NPs by VSP4 under solid-state fermentation.

In recent years, gold nanoparticles (Au-NPs) have garnered popularity for their remarkable and promising applications in various areas. Here, we report the synthesis of Au-NPs using extracellular amylase produced by VSP4 under solid-state fermentation (SSF) through the reduction of AuCl with retention of enzymatic activity in the complex. Accordingly, VSP4 was exploited to enhance α-amylase production under SSF using the Plackett-Burman design, followed by the central composite design (CCD) of response surface methodology (RSM).

Application of statistical methodology for the optimization of L-glutaminase enzyme production from Streptomyces pseudogriseolus ZHG20 under solid-state fermentation.

Background: Actinomycetes are excellent microbial sources for various chemical structures like enzymes, most of which are used in pharmaceutical and industrial products. Actinomycetes are preferred sources of enzymes due to their high ability to produce extracellular enzymes. L-glutaminase has proven its essential role as a pharmaceutical agent in cancer therapy and an economic agent in the food industry.

Decoding the microbial universe with metagenomics: a brief insight.

A major part of any biological system on earth involves microorganisms, of which the majority are yet to be cultured. The conventional methods of culturing microbes have given fruitful outcomes yet have limitations. The curiosity for better understanding has led to the development of culture-independent molecular methods that help push aside the roadblocks of earlier methods.

Optimization of biodegradable cross-linked guar-gum-PLA superabsorbent hydrogel formation employing response surface methodology.

Cross-linked polymer networks with three-dimensional structures known as hydrogels absorb and retain a large amount of water. Because of their properties, hydrogel materials have been considered a boon in agriculture science. In the present investigation, guar gum cross-linked polylactic acid hydrogel is synthesized using MMA as monomer and optimized using a central composite design of response surface methodology for better swelling.

Comparative Genome Analysis of Focusing on Phylogenomics, Functional Traits, and Prevalence of Antimicrobial and Virulence Genes.

is a gram-positive, nonpathogenic, endospore-forming, member of a group of free-living soil bacteria with a variety of traits including plant growth promotion, production of antifungal and antibacterial metabolites, and production of industrially important enzymes. We have attempted to reconstruct the biogeographical structure according to functional traits and the evolutionary lineage of using comparative genomics analysis. All the available 96 genomes of strains were curated from the NCBI genome database, having a variety of important functionalities in all sectors keeping a high focus on agricultural aspects.

Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing strain KCP2.

strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic α-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries.

The effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using Mehsani buffalo (Bubalus bubalis).

In developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. It is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. A metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic Mehsani buffalo (Bubalus bubalis) fed with varying proportions of roughage.