Multiplexed measurement of proteins in tissue in a clinical environment.

There is an emerging need for more effective approaches to accurately quantitate protein expression in tissue samples. In many clinical studies and particularly in pharmaceutical clinical trials, access to adequate tissue samples is a major bottleneck, and thus techniques to measure protein expression in these valuable tissue specimens is important. This study will review current approaches in multiplexing of protein expression in tissue, and discusses new approaches using a novel image registration technique across multiple tissue sections.