Publications by authors named "Tin Tin Manh Nguyen"
Article Synopsis
- The gut microbiome plays a crucial role in how drugs are metabolized and how effective they are, but there hasn't been an effective way to monitor these changes without using labels.
- A new 2D nuclear magnetic resonance (NMR) technique allows real-time monitoring of the metabolism of the Parkinson's drug Levodopa in live bacteria and in symbiotic systems with the host organism Caenorhabditis elegans.
- The study finds that different strains of the same bacterial species, Enterococcus faecalis, produce varying amounts of dopamine despite having the same gene for L-dopa metabolism, indicating that gene presence doesn't always reflect metabolic activity; this new method could be applied to other drugs to understand how they interact with the
Background And Aims: SKN-1, a C. elegans transcription factor analogous to the mammalian NF-E2-related factor (Nrf2), has been known to promote oxidative stress resistance aiding nematodes' longevity. Although SKN-1's functions suggest its implication in lifespan modulation through cellular metabolism, the actual mechanism of how metabolic rearrangements contribute to SKN-1's lifespan modulation has yet to be well characterized.
View Article and Find Full Text PDFGPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT.
J Exp Clin Cancer Res
February 2023
Background: Clear cell renal cell carcinoma (ccRCC), with its hallmark phenotype of high cytosolic lipid content, is considered a metabolic cancer. Despite the implication of this lipid-rich phenotype in ccRCC tumorigenesis, the roles and regulators of de novo lipid synthesis (DNL) in ccRCC remain largely unexplained.
Methods: Our bioinformatic screening focused on ccRCC-lipid phenotypes identified glutathione peroxidase 8 (GPX8), as a clinically relevant upstream regulator of DNL.
Gut microbiome can affect drug metabolism considerably, leading to modified drug response. However, quantitative estimation of host vs. microbial contributions in a living host-gut microbiome system has been challenging.
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- Mitochondrial fatty acid oxidation (FAO) plays a crucial role in cancer metabolism, yet the mechanisms behind it and therapeutic strategies targeting catabolic metabolism are still unclear.
- Snail (SNAI1), a key regulator of epithelial-mesenchymal transition, promotes catabolic FAO, helping breast cancer cells survive in low-nutrient conditions by suppressing mitochondrial ACC2.
- Inhibiting both the pentose phosphate pathway and FAO with existing drugs can reverse the metabolic shifts caused by Snail, potentially hindering the spread of breast cancer.
AMP-activated protein kinase (AMPK in human and AAK in ) is a master regulator of metabolism. It has many isotypes, but its isotype-dependent functions are largely unknown. By developing real-time in-organism NMR metabolomics for , we were able to study different roles of the isotypic catalytic subunits of AAK/AMPK, AAK-1, and AAK-2 in live worms at the whole organism level.
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