Molecular Insights into the Interaction between CD147 and the SARS-CoV‑2 Spike Protein.

Cluster of differentiation 147 (CD147), a transmembrane glycoprotein, has been identified as a potential auxiliary receptor for the SARS-CoV-2 spike protein (SP), contributing to COVID-19 infection. However, the detailed binding characteristics of this interaction remain unclear. Here, we characterized SP-CD147 binding using Enzyme-Linked Immunosorbent Assay (ELISA) and single-molecule force spectroscopy (SMFS) under varying contact times and temperatures.