Histological and histomorphometric aspects of continual intermittent parathyroid hormone administration on osseointegration in osteoporosis rabbit model.

In implant treatment, primary stability and osseointegration are improved by continual intermittent administration of parathyroid hormone (PTH) in patients with osteoporosis. However, the histological and histomorphometric aspects are not clear. The aim of this study was to investigate the histological and histomorphometric effects of intermittent PTH administration on osseointegration in a glucocorticoid-induced osteoporotic rabbit model.

Osseointegration Aspects of Implants at the Bone Reconstruction Site by a Novel Porous Titanium Scaffold.

Objectives: Porous titanium is used for the reconstruction of large bone defects due to its excellent mechanical strength. The quality of osseointegration of implants placed in bone reconstructed with porous titanium is unknown. The purpose of this study was to evaluate the osseointegration of implants at sites reconstructed using porous titanium.

Generalized periodontitis treated with periodontal, orthodontic, and prosthodontic therapy: A case report.

Background: Generalized periodontitis is a severe periodontal disease characterized by rapid periodontal destruction in healthy persons. This case report describes the treatment of a severe crowding, large overjet, and occlusal collapse due to the loss of anterior guidance with generalized periodontitis.

Case Summary: A 35-year-old female patient with a chief complaint of crowding and maxillary protrusion was diagnosed with generalized periodontitis by clinical and radiographic examinations.

Comparative Study of Surface Modification Treatment for Porous Titanium.

Objectives: This study was to investigate suitable surface treatment methods for porous titanium by study of material properties and calcium phosphate deposition in simulated body fluid.

Material And Methods: Porous titanium with acid (HSO and HCl mixed acid) or alkali (NaOH) treatment was prepared. The surfaces were observed, and the weight change ratio (after and before surface treatment) and compression strength were measured.

The development of novel bioactive porous titanium as a bone reconstruction material.

Porous titanium fabricated by the resin-impregnated titanium substitute technique has good mechanical strength and osteoconduction. The alkali treatment of the titanium surface creates a bioactive surface. Alkali-treated porous titanium is expected to accelerate bone formation.

Development of a novel bioactive titanium membrane with alkali treatment for bone regeneration.

This study evaluates a bioactive titanium membrane with alkali treatment for stimulating apatite formation and promoting bone regeneration. The titanium thin membranes were either treated with NaOH (alkali-group) or untreated (control). Each sample were incubated in simulated body fluid.

Novel fabrication of porous titanium by a resin-impregnated titanium substitution technique for bone reconstruction.

The purpose of this study was to develop a novel porous titanium material with superior mechanical strength and osteoconduction for bone reconstruction. Porous titanium samples were fabricated by titanium-slurry impregnate to prepare urethane forms with several porosities (high-porosity; 92%, middle-porosity; 85% and low-porosity; 65%). Porous HA (mean porosity; 75.

Enhancement of calcification by osteoblasts cultured on hydroxyapatite surfaces with adsorbed inorganic polyphosphate.

Inorganic polyphosphate has been expected to accelerate bone regeneration. However, there are limited evidences to prove that polyphosphate adsorbed on the surface of a hydroxyapatite plate enhances calcification of cultured osteoblasts. In this study, we examined the effect of polyphosphate adsorbed onto the surface of a hydroxyapatite plate on the attachment, proliferation, differentiation, and calcification of osteoblasts.

Effects of continual intermittent administration of parathyroid hormone on implant stability in the presence of osteoporosis: an in vivo study using resonance frequency analysis in a rabbit model.

Objective: This study aimed to evaluate the effects of continual intermittent administration of parathyroid hormone (PTH) on implant stability in the presence of osteoporosis, using rabbit models.

Material And Methods: Fifteen female New Zealand white rabbits underwent ovariectomy and were administered glucocorticoids to induce osteoporosis, following which they were divided into three groups. The first group received intermittent subcutaneous PTH for 4 weeks until implant placement (PTH1), while the second and third groups received PTH (PTH2) and saline (control), respectively, for 4 weeks before and after implant placement.

A stability evaluation of a novel titanium dental implant/interconnected porous hydroxyapatite complex under functional loading conditions.

The aim of this study was to evaluate the stability of implant/interconnected porous calcium hydroxyapatite complex (implant/IPCHA-complex) under functional loading. Implant/IP-CHA-complexes were placed into the mandibles of four Beagle-Labrador hybrid dogs (complex-group). On the other side, an implant was placed directly (control-group).

Osseointegration aspects of placed implant in bone reconstruction with newly developed block-type interconnected porous calcium hydroxyapatite.

Objectives: The purpose of this study was to evaluate the osseointegration of dental implant in bone reconstructions with interconnected porous calcium hydroxyapatite (IP-CHA).

Material And Methods: The IP-CHA cylinders (D; 4.3 mm, H; 10.

A novel mouthpiece prevents bite injuries caused by intraoperative transcranial electric motor-evoked potential monitoring.

Purpose: Intraoperative transcranial motor-evoked potential monitoring causes contraction of the masseter muscles, which may cause injuries to the oral cavity and damage to the orotracheal tube. We developed a mouthpiece made from vinyl-silicone impression material to prevent these injuries. The purpose of this study was to examine its efficacy and safety.

Intermittent administration of parathyroid hormone enhances primary stability of dental implants in a bone-reduced rabbit model.

The aim of the study was to investigate the effect of parathyroid hormone (PTH) on primary stability of dental implants in a bone-reduced model. Ten female New Zealand white rabbits underwent ovariectomy and were administered glucocorticoid to induce osteoporosis. One group was administered PTH intermittently by subcutaneous injection for 4 weeks (PTH-group) and the other group was given injections of saline for 4 weeks (Osteoporosis; OP-group).

Influence of implant surface topography on primary stability in a standardized osteoporosis rabbit model study.

Evaluating primary stability is important to predict the prognosis of dental implant treatment. Primary stability is decreased in a low bone density site such as osteoporosis. However, it is difficult to apply in small animal and the effect of the different implant surface topography for the primary stability at low bone density site has not yet fully been investigated.

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: an animal study.

Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control).

Polyphosphate-mediated inhibition of tartrate-resistant acid phosphatase and suppression of bone resorption of osteoclasts.

Inorganic polyphosphate (poly(P)) has recently been found to play an important role in bone formation. In this study, we found that tartrate-resistant acid phosphatase (TRAP), which is abundantly expressed in osteoclasts, has polyphosphatase activity that degrades poly(P) and yields Pi as well as shorter poly(P) chains. Since the TRAP protein that coprecipitated with anti-TRAP monoclonal antibodies exhibited both polyphosphatase and the original phosphatase activity, poly(P) degradation activity is dependent on TRAP and not on other contaminating enzymes.

Inorganic polyphosphate suppresses lipopolysaccharide-induced inducible nitric oxide synthase (iNOS) expression in macrophages.

In response to infection, macrophages produce a series of inflammatory mediators, including nitric oxide (NO), to eliminate pathogens. The production of these molecules is tightly regulated via various mechanisms, as excessive responses are often detrimental to host tissues. Here, we report that inorganic polyphosphate [poly(P)], a linear polymer of orthophosphate ubiquitously found in mammalian cells, suppresses inducible nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS), a cell wall component of Gram-negative bacteria, in mouse peritoneal macrophages.

New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering.

Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs200 was larger than that on the non-desalinated ChSs200.

Development of implant/interconnected porous hydroxyapatite complex as new concept graft material.

Background: Dental implant has been successfully used to replace missing teeth. However, in some clinical situations, implant placement may be difficult because of a large bone defect. We designed novel complex biomaterial to simultaneously restore bone and place implant.

Comparative evaluation of bone regeneration using spherical and irregularly shaped granules of interconnected porous hydroxylapatite. A beagle dog study.

Purpose: Bone regeneration stimulated by two different shapes of interconnected porous calcium hydroxyapatite (IP-CHA) granules was evaluated in the mandibles of 3 beagle dogs.

Methods: Deferent shapes of IP-CHA were used, spherical (spherical shaped IP-CHA granules) and irregular shapes (irregularly shaped IP-CHA granules). Two bone sockets (3mm in diameter and 5mm in depth) were prepared in the right edentulous mandible of each animal where right premolars had been extracted and sites healed for 3 months.

Bone formation ability of carbonate apatite-collagen scaffolds with different carbonate contents.

Hydroxyapatite and carbonate apatites with different carbonate contents were synthesized, mixed with atelocollagen, and made into sponge scaffolds. The scaffolds were implanted into the bone sockets of the femurs of male New Zealand white rabbits for 2, 3, 12 and 24 weeks. carbonate apatite-collagen scaffold with 4.

Initial bone regeneration around fenestrated implants in Beagle dogs using basic fibroblast growth factor-gelatin hydrogel complex with varying biodegradation rates.

Purpose: The purpose of this study was to compare the effectiveness of fast and slow biodegradation of basic fibroblast growth factor (bFGF)-gelatin hydrogel complex on bone regeneration around fenestrated implants as a new augmentation drug delivery system.

Methods: Nine titanium implants (3.3mm diameter and 10mm length) were placed into the edentulous areas of the mandibles of three adult beagle dogs with four screws exposed at the upper buccal side.

Influence of static overload on the bony interface around implants in dogs.

Purpose: The aim of this study was to investigate bony changes around selectively overloaded implants in dogs.

Materials And Methods: Twelve adult male beagles were used: 4 animals each for control, 4-week-load, and 12-week-load groups. Three implants were placed on the right side of each of the 12 animals and the time point designated as the study's baseline.

Development of new drug delivery system for implant bone augmentation using a basic fibroblast growth factor-gelatin hydrogel complex.

This study sought to clarify the effectiveness of bFGF-gelatin hydrogel complex on bone regeneration around implants for the development of a new drug delivery system for bone augmentation. Twenty-four titanium implants (03.3 mmX 10 mm) were placed into edentulous areas of the mandibles of four beagle dogs with the upper four screw threads exposed at buccal side.

Development of cell-hybrid artificial bone: effect of osteogenic differentiation of bone marrow stromal stem cells on bone formation with newly developed interconnected porous calcium hydroxyapatite.

The purpose of this study was to evaluate the effect of osteogenic differentiation of bone marrow stromal stem cells (BMSCs) on bone formation in a novel interconnected porous calcium hydroxyapatite (IP-CHA). BMSCs/IP-CHA composites, as a cell-hybrid artificial bone, were made by injecting BMSCs solution into IP-CHA scaffolds. To induce osteogenic differentiation, BMSCs/IP-CHA composites were subcultured for three, seven, 10, and 14 days.