Chloroplast DNA markers for distinguishing seaweeds and using real-time quantitative PCR and their application in commercial products.

Unlabelled: and , popular seaweed foods in Korea, get frequently mixed in commercial food products because of their overlapping growing locations, seasons, and morphological characteristics. In this study, molecular markers were developed to distinguish these species using a real-time PCR. Six primer pairs were designed for chloroplast genes , , and .

Complete organellar genomes of six Sargassum species and development of species-specific markers.

Sargassum is one of the most important brown algal genera that can be used as food and raw material for medicinal purpose, and has various beneficial effects. As the classification of Sargassum species is currently based on their morphological characteristics, organellar genome sequences of Sargassum would provide important information for accurate identification of species and developing species-specific markers. We sequenced the complete organellar genomes of six Sargassum species, including the first complete chloroplast genome sequences of S.

The complete mitochondrial genome of (Bangiales, Rhodophyta).

(Kjellman) L.-E. Yang & J.

An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species.

The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid and mitochondrial gene sequences to distinguish the six representative species produced via mariculture in Korea. The , , and sequences of 15 species from the NCBI database were aligned to determine specific restriction enzyme sites of the six species. To confirm the presence of restriction sites of eight enzymes, PCR amplicons were digested as follows: a 556 bp fragment within the region of chloroplast DNA was confirmed in using I, whereas 111I, II, I, and AI enzymes produced fragments of 664, 271, 600, and 510 bp, respectively, from the region of mitochondrial DNA in , , , and .