TAL1 overexpression in induced pluripotent stem cells promotes the formation of hematopoietic cell-forming complexes but inhibits enucleation .

The generation of human red blood cells (RBCs) from stem cells, such as induced pluripotent stem cells (iPSCs), holds promise for transfusable RBCs but faces challenges, including RBC maturation, enucleation, and large-scale production. In this study, we evaluated the effect of conditional TAL1 overexpression on RBC production via hematopoietic cell-forming complex (HCFC) formation from iPSCs because is a key regulatory transcription factor essential for erythropoiesis. overexpression in iPSCs, either before or after hematopoietic induction, significantly enhanced HCFC formation and hematopoietic differentiation, as evidenced by increased hematopoiesis-related gene expression, a higher yield of glycophorin A (GPA)+/CD71+ cells, and elevated gamma hemoglobin levels.

Establishment of an erythroid differentiation system from canine peripheral blood mononuclear cells.

Blood transfusion is a critical, lifesaving medical procedure for dogs. However, the limited availability of blood donors and ethical concerns highlight the need for alternative solutions, such as -produced red blood cells (RBCs), which remain unexplored in canines. This study aimed to produce canine erythrocytes from peripheral blood (PB) mononuclear cells (MNCs), optimize culture conditions using either human or canine reagents, and identify relevant cell markers.