A multiplexed LC-MS/MS method to reveal changes in inflammatory and coagulation cascades induced by host infection.

Inflammation has been considered a pivotal player in the most severe forms of respiratory infections like COVID-19, which induces a significant alteration of inflammatory cytokines called cytokine storm. Above the immune system, abnormalities in coagulation parameters have been observed in COVID-19-affected subjects displaying an increased risk of a blood clot causing thromboembolic events. Currently, clinical methods for monitoring infections rely on serological tests that detect the antibody response using enzyme-linked immunosorbent assays (ELISA).

The impact of glyphosate at regulatory "safe" levels on reproductive health: cellular and molecular disruptions on male germ line.

Glyphosate, a widely used herbicide in both agricultural and non-agricultural practices, has become pervasive in the environment, leading to significant human and animal exposure. Despite growing evidence of its potential endocrine-disrupting and reproductive toxicity, European regulatory agencies continue to affirm its safety. This study examines the effects of glyphosate on male reproductive health by exposing adult zebrafish to dietary glyphosate at doses within European safety thresholds.

Rapid modulation of interscapular brown adipose tissue mitochondrial activity by ketosis induced by 1,3-butanediol administration to rats.

Some studies indicate that brown adipose tissue (BAT) represents a promising target in the fight against dysmetabolic diseases, with indications suggesting it as a potential target for the effects of ketone bodies. We investigate whether the elevation of plasma levels of the ketone body β-hydroxybutyrate, achieved through the in vivo administration of its precursor 1,3-butanediol (BD) to rats, could impact interscapular BAT (iBAT) mitochondrial biochemistry and functionality. We examined the effects induced by BD within 3 h and after 2 weeks of treatment.

Determination of Fertility Hormones and Adipokines by LC-MRM/MS Analysis.

Due to the increase in the rate of male and female infertility, assisted fertilization practices are currently adopted as valid support for couples unable to get pregnant. Analytical approaches for fertility hormone dosages are constantly being developed, following the technological progress of fertilization methods that have evolved for more than a century. Indeed, the analysis of fertility hormones in serum samples is a common clinical practice to check the fertility state, but absolute quantification of these hormones is a great challenge due to biological variability and low serum concentrations.