Carboxylation capacity is the main limitation of carbon assimilation in High Arctic shrubs.

Increases in shrub height, biomass and canopy cover are key whole-plant features of warming-induced vegetation change in tundra. We investigated leaf functional traits underlying photosynthetic capacity of Arctic shrub species, particularly its main limiting processes such as mesophyll conductance. In this nutrient-limited ecosystem, we expect leaf nitrogen concentration to be the main limiting factor for photosynthesis.

When and where soil is important to modify the carbon and water economy of leaves.

Photosynthetic 'least-cost' theory posits that the optimal trait combination for a given environment is that where the summed costs of photosynthetic water and nutrient acquisition/use are minimised. The effects of soil water and nutrient availability on photosynthesis should be stronger as climate-related costs for both resources increase. Two independent datasets of photosynthetic traits, Globamax (1509 species, 288 sites) and Glob13C (3645 species, 594 sites), were used to quantify biophysical and biochemical limitations of photosynthesis and the key variable C /C (CO drawdown during photosynthesis).

Production of Biopharmaceuticals in -Axillary Stem Growth as a Key Determinant of Total Protein Yield.

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale.

Organic Nitrogen Uptake and Assimilation in Using Position-Specific Labeling and Compound-Specific Isotope Analysis.

Organic nitrogen is now considered a significant source of N for plants. Although organic management practices increase soil organic C and N content, the importance of organic N as a source of crop N under organic farming management systems is still poorly understood. While dual-labeled (C and N) molecule methods have been developed to study amino acid uptake by plants, multiple biases may arise from pre-uptake mineralization by microorganisms or post-uptake metabolism by the plant.

Thermal acclimation of photosynthesis and respiration of southern and northern white spruce seed sources tested along a regional climatic gradient indicates limited potential to cope with temperature warming.

Background And Aims: Knowledge of thermal acclimation of physiological processes of boreal tree species is necessary to determine their ability to adapt to predicted global warming and reduce the uncertainty around the anticipated feedbacks of forest ecosystems and global carbon cycle to climate change. The objective of this work was to examine the extent of thermal acclimation of net photosynthesis (An) and dark respiration (Rd) of two distant white spruce (Picea glauca) seed sources (from south and north of the commerial forest zone in Québec) in response to latitudinal and seasonal variations in growing conditions.

Methods: The temperature responses of An, its biochemical and biophysical limitations, and Rd were measured in 1-year-old needles of seedlings from the seed sources growing in eight forest plantations along a regional thermal gradient of 5.

Persistent negative temperature response of mesophyll conductance in red raspberry (Rubus idaeus L.) leaves under both high and low vapour pressure deficits: a role for abscisic acid?

The temperature dependence of mesophyll conductance (g ) was measured in well-watered red raspberry (Rubus idaeus L.) plants acclimated to leaf-to-air vapour pressure deficit (VPDL) daytime differentials of contrasting amplitude, keeping a fixed diurnal leaf temperature (T ) rise from 20 to 35 °C. Contrary to the great majority of g temperature responses published to date, we found a pronounced reduction of g with increasing T irrespective of leaf chamber O level and diurnal VPDL regime.

Greater efficiency of water use in poplar clones having a delayed response of mesophyll conductance to drought.

Improvement of water use efficiency is a key objective to improve the sustainability of cultivated plants, especially fast growing species with high water consumption like poplar. It is well known that water use efficiency (WUE) varies considerably among poplar genotypes, and it was recently suggested that the use of the mesophyll-to-stomatal conductance ratio (gm/gs) would be an appropriate trait to improve WUE. The responses of 7-week-old cuttings of four hybrid poplar clones and one native Balsam poplar (Populus balsamifera L.

Threshold response of mesophyll CO2 conductance to leaf hydraulics in highly transpiring hybrid poplar clones exposed to soil drying.

Mesophyll conductance (gm) has been shown to impose significant limitations to net CO2 assimilation (A) in various species during water stress. Net CO2 assimilation is also limited by stomatal conductance to water (gsw), both having been shown to co-vary with leaf hydraulic conductance (Kleaf). Lately, several studies have suggested a close functional link between Kleaf, gsw, and gm.

Variable mesophyll conductance revisited: theoretical background and experimental implications.

The CO(2) concentration at the site of carboxylation inside the chloroplast stroma depends not only on the stomatal conductance, but also on the conductance of CO(2) between substomatal cavities and the site of CO(2) fixation. This conductance, commonly termed mesophyll conductance (g(m) ), significantly constrains the rate of photosynthesis. Here we show that estimates of g(m) are influenced by the amount of respiratory and photorespiratory CO(2) from the mitochondria diffusing towards the chloroplasts.

Construction costs, chemical composition and payback time of high- and low-irradiance leaves.

The effect of irradiance on leaf construction costs, chemical composition, and on the payback time of leaves was investigated. To enable more generalized conclusions, three different systems were studied: top and the most-shaded leaves of 10 adult tree species in a European mixed forest, top leaves of sub-dominant trees of two evergreen species growing in small gaps or below the canopy in an Amazonian rainforest, and plants of six herbaceous and four woody species grown hydroponically at low or high irradiance in growth cabinets. Daily photon irradiance varied 3-6-fold between low- and high-light leaves.

Carbon flux and growth in mature deciduous forest trees exposed to elevated CO2.

Whether rising atmospheric carbon dioxide (CO2) concentrations will cause forests to grow faster and store more carbon is an open question. Using free air CO2 release in combination with a canopy crane, we found an immediate and sustained enhancement of carbon flux through 35-meter-tall temperate forest trees when exposed to elevated CO2. However, there was no overall stimulation in stem growth and leaf litter production after 4 years.

Elevated CO2 reduces sap flux in mature deciduous forest trees.

We enriched in CO2 the canopy of 14 broad-leaved trees in a species-rich, ca. 30-m-tall forest in NW Switzerland to test whether elevated CO2 reduces water use in mature forest trees. Measurements of sap flux density (JS) were made prior to CO2 enrichment (summer 2000) and throughout the first whole growing season of CO2 exposure (2001) using the constant heat-flow technique.

Web-FACE: a new canopy free-air CO2 enrichment system for tall trees in mature forests.

The long-term responses of forests to atmospheric CO2 enrichment have been difficult to determine experimentally given the large scale and complex structure of their canopy. We have developed a CO2 exposure system that uses the free-air CO2 enrichment (FACE) approach but was designed for tall canopy trees. The system consists of a CO2-release system installed within the crown of adult trees using a 45-m tower crane, a CO2 monitoring system and an automated regulation system.