DendriPep Nanocoats: Substrate-Agnostic, Self-Assembling Constructs with Shear-Controlled Thickness and Permeability.

This study investigates the self-assembly of hybrid poly(amidoamine)-peptide dendrimers (DendriPeps) into shear-responsive vesicle-like structures with nanometric thickness, called "Nanocoats", that are capable of encapsulating nano- and microscale particles. To assess the material-agnostic coating power of DendriPeps, we tested the formation of Nanocoats on a variety of synthetic and biological substrates, including polystyrene nanoparticles, poly(-isopropylacrylamide) microgels, gallium-indium liquid metal nanodroplets, and bacteriophages and lentiviruses. Specifically, we utilized spectroscopic and microscopic techniques to monitor the reversible assembly of Nanocoats on the surface of the particles upon controlling the shear stress of the surrounding aqueous phase.

Advances and opportunities in process analytical technologies for viral vector manufacturing.

Viral vectors are an emerging, exciting class of biologics whose application in vaccines, oncology, and gene therapy has grown exponentially in recent years. Following first regulatory approval, this class of therapeutics has been vigorously pursued to treat monogenic disorders including orphan diseases, entering hundreds of new products into pipelines. Viral vector manufacturing supporting clinical efforts has spurred the introduction of a broad swath of analytical techniques dedicated to assessing the diverse and evolving panel of Critical Quality Attributes (CQAs) of these products.

Enrichment of adeno-associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients.

Adeno-associated virus-based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without the encapsidated gene of interest. Capsids devoid of the gene of interest, or "empty" capsids, represent a product-related impurity.

Towards Resorbable Elastomeric Circuit Boards for Implantable Medical Devices.

IMDs are typically considered for chronic-use applications and a limited set of implant locations. Resorbable IMDs seek to combine advances in flexible electronics with functional soft materials to enable new applications, including acute care, aiming at temporary interfacing with soft tissues. Poly(oc-tamethylene maleate (anhydride) citrate) (POMaC) is an elasto-mer with demonstrated high biocompatibility and bioresorbability, as well as tunable stiffness and surface properties.

Nonwoven Membranes with Infrared Light-Controlled Permeability.

This study presents the development of the first composite nonwoven fiber mats (NWFs) with infrared light-controlled permeability. The membranes were prepared by coating polypropylene NWFs with a photothermal layer of poly(-isopropylacrylamide) (PNIPAm)-based microgels impregnated with graphene oxide nanoparticles (GONPs). This design enables "photothermal smart-gating" using light dosage as remote control of the membrane's permeability to electrolytes.

Modified gaphene oxide (GO) particles in peptide hydrogels: a hybrid system enabling scheduled delivery of synergistic combinations of chemotherapeutics.

The scheduled delivery of synergistic drug combinations is increasingly recognized as highly effective against advanced solid tumors. Of particular interest are composite systems that release a sequence of drugs with defined kinetics and molar ratios to enhance therapeutic effect, while minimizing the dose to patients. In this work, we developed a homogeneous composite comprising modified graphene oxide (GO) nanoparticles embedded in a Max8 peptide hydrogel, which provides controlled kinetics and molar ratios of release of doxorubicin (DOX) and gemcitabine (GEM).