Separation-free cholesterol detection using an enzyme-doped hydrogel opto-microfluidic hybrid chip via colorimetry and light extinction spectroscopy.

Cholesterol levels serve as a critical biomarker for the diagnosis of cardiovascular diseases. However, conventional detection methods are often time-consuming and reliant on specialized laboratory equipment. In this study, we present a novel microfluidic lab-on-a-chip platform designed for the rapid and accurate detection of cholesterol, employing an enzyme-doped agarose hydrogel combined with colorimetric analysis.

Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.

Loop-mediated isothermal amplification (LAMP) is a rapid, state-of-the-art DNA amplification technology, used primarily for the quick diagnosis and early identification of microbial infection, caused by pathogens such as virus, bacteria and malaria. A target DNA can be amplified within 30 min using the LAMP reaction, taking place at a steady temperature. The LAMP method uses four or six primers to bind eight regions of a target DNA and has a very high specificity.

Structural and Surface Properties of pH-Varied FeO Nanoparticles: Correlation with Antibacterial Properties.

Hematite (FeO) nanoparticles were synthesized using a hydrothermal synthesis route under different pH conditions (pH ∼8,10,11.5) (i.e.