Peri-follicular blood flow in the follicle from which ovulation occurs in cows.

The hypothesis of the study was that the vascularity of ovulatory follicles (OF) determines the ovulatory status. The peri-follicular blood flow characteristics of OF were sequentially recorded using Color Doppler Imaging ultrasonography from the onset of induced estrus (Day 0) to ovulation at 12 h intervals. The OFs were categorized into two groups based on the timing of ovulation (i) Normal ovulation (OFN) - When ovulation occurred within 36 h after the onset of estrus (n = 18) and (ii) Delayed ovulation (OFD) - When ovulation occurred after 36 h (n = 15).

Evaluation of pheromone-based kit: A noninvasive approach of estrus detection in buffalo.

In view of the silent nature of estrus in buffalo, a noninvasive assay kit has long been felt necessary for easy and effective estrus detection. This study was designed to detect estrus in buffalo using a kit formulated in our laboratory based on pheromone compound. Group I: Urine samples collected at estrus phase and group II: randomly collected urine samples were subjected to the test using the kit.