Synthesis of terbium-labelled tetracycline-loaded calcium phosphate nanoparticle and its mode of action on multi-drug-resistant pathogenic bacteria Escherichia coli and Salmonella kentucky.

This study dealt with synthesis of a luminescent nano-form of tetracycline, characterization of its important physico-chemical properties, and molecular mechanism of its antibacterial action on tetracycline-resistant bacterial species. Nanonization was done by entrapping tetracycline (Tet) molecules within calcium phosphate nanoparticles (CPNPs) and doping them with fluorescent terbium (Tb) ions. To characterize the particles, techniques like AFM, SEM, TEM, DLS, absorption-fluorescence-FTIR spectrometry and dialysis were used and to investigate their antibacterial potency and mechanism of action, techniques of agar plating, Ni-NTA chromatography, absorption-fluorescence-CD spectroscopy, gel electrophoresis and NS-TEM were used.

Silver nano-colloid particles embedded on Langmuir-Blodgett film matrix of stearic acid serving as a SERS active sensor for detecting the herbicide 'Paraquat'.

The herbicide Paraquat, widely used for efficient weed control, poses significant health risks to humans viz., severe toxicity to vital organs and induction of neurodegenerative disorder like Parkinson's disease, underscoring the urgent need for developing sensitive detection methods for the herbicide. This study aims at fabricating a novel SERS-active substrate SA-LB/Ag (silver nano-colloids adsorbed on Langmuir-Blodgett film of stearic acid), as a SERS based sensor having high sensitivity, uniformity, and reproducibility to detect ultra-trace amounts of paraquat.

Two new oligomers of E. coli small heat-shock protein IbpB identified under heat stress exhibit maximum holding chaperone activity.

Escherichia coli small heat-shock protein IbpB (MW: 16 KDa) has holding chaperone activity and is present in cells at 30 °C as two large oligomers of MW 2.0-3.0 MDa and 600-700 KDa.

Synthesis of novel hydrophilic celastrol nanoformulation by entrapment within calcium phosphate nanoparticle and study of its antioxidant activity against neurotoxin-induced damage in human neuroblastoma cells.

Celastrol, a pentacyclic triterpenoid found in Chinese herb Tripterygium wilfordii, is considered as one of the top-five natural medicinal compounds with high antioxidant property. However, celastrol has poor aqueous solubility and thereby low bioavailability, restricting its clinical application as drug. To overcome this problem, we nanonized celastrol by entrapping it within hydrophilic nanocarrier - calcium phosphate nanoparticle.

Antibiofilm potential of nanonized eugenol against Pseudomonas aeruginosa.

Aims: The purpose of this study was to synthesize a nanoform of eugenol (an important phytochemical with various pharmacological potentials) and to investigate its antibiofilm efficacy on Pseudomonas aeruginosa biofilm.

Methods And Results: Colloidal suspension of eugenol-nanoparticles (ENPs) was synthesized by the simple ultrasonic cavitation method through the emulsification of hydrophobic eugenol into hydrophilic gelatin. Thus, the nanonization process made water-insoluble eugenol into water-soluble nano-eugenol, making the nanoform bioavailable.