Magnetite-enhanced chain elongation via endogenous electron donors through a novel fungi-bacteria microbiome.

Anaerobic fermentation of organic waste stream into medium-chain fatty acids (MCFA) through chain elongation (CE) has emerged as a sustainable and eco-friendly approach for resource recovery. Co-culture of yeast with chain elongator achieved a promising endogenous electron donor (ED)-driven CE but was limited by the weak yeast-bacteria synergy. This study presents a novel approach to optimizing the CE process through the regulation of magnetite (0-15 g/L).

Unveiling the bioelectrocatalyzing behaviors and microbial ecological mechanisms behind caproate production without exogenous electron donor.

Bioelectrochemical systems were proposed as a promising approach for the efficient valorization of biomass into 6-8 carbon atom medium-chain fatty acids (MCFAs), the precursors for high value-added chemicals or renewable energy, via acetyl-CoA-mediated chain elongation (CE). To achieve CE processes, exogenous electron donors (EDs), e.g.

Elucidating the microbial ecological mechanisms on the electro-fermentation of caproate production from acetate via ethanol-driven chain elongation.

Electro-fermentation (EF) is an attractive way to implement the chain elongation (CE) process, by controlling the fermentation environment and reducing the dosage of external electron donors (EDs). However, besides the coexistence performance of external EDs and electrode, applications of EF technology on the fermentation broth containing both EDs and electron acceptors during CE process, are all still limited. The current study investigated the contribution of EF to caproate production, under different acetate: ethanol ratios (R).

Quorum sensing shaped microbial consortia and enhanced hydrogen recovery from waste activated sludge electro-fermentation on basis of free nitrous acid treatment.

In this study, the feasibility of free nitrous acid (FNA) pretreatment coupled with quorum sensing (QS) was investigated to enhance hydrogen recovery from waste activated sludge (WAS) via electro-fermentation (EF). 3-oxo-hexanoyl-homoserine lactone (3OC6-HSL), as the signal molecule, was only added in the first three cycles of sludge inoculation at the phase of microbial electrolysis cells (MECs) startup. Results showed that QS combined FNA (AHL-FMEC) enabled highest hydrogen yield and current (4.