Utilizing the Laparoscopic Ovum Pick-Up Method for In Vitro Embryo Production in Prepubertal Anatolian Native Black Calves: A Promising Approach for Gene Banks and Endangered Species.

Recent advancements in genomic technology have increased interest in using calves as oocyte sources through the laparoscopic ovum pick-up (LOPU) method, which shortens generation intervals and enhances genetic gain. This study aimed to produce embryos in vitro using oocytes from endangered Anatolian Native Black calves and to increase the number of embryos in the gene bank through LOPU, a method not previously studied in Turkey. Four Anatolian Native Black calves, approximately 3 months old, were used.

Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.

ProAKAP4 is a sperm structural protein that regulates motility through the PKA-dependent cAMP signaling pathway, which is synthesized as an X chromosome-linked member of the gene family. This study aims to determine the optimal level of proAKAP4 for evaluating sexed semen through investigating its relationship with the longevity of sperm quality in sexed Holstein bull sperm. A total of 30 sexed sperm samples (bearing X chromosomes) from 30 distinct Holstein bulls ( = 30) were analyzed.

Reaction time as a libido indicator and its relation to pre-freeze and post-thaw sperm quality in bulls.

Libido and sperm quality output relationship is already not clear in farm animals. The present study compared reaction time (RT) as a libido indicator and the pre-freeze and post-thaw sperm quality of AI bulls. Before the collection of ejaculates (n = 53, from 22 AI bulls [4.