A red fluorescent probe with new recognition site for tracking the fluctuation of biothiol in drug-induced liver injury model.

Background: Drug-induced liver injury (DILI), which results from pharmaceuticals or their metabolites, is intricately associated with oxidative stress and is the most common liver disease. Biothiols play pivotal roles in maintaining redox homeostasis, cellular signalling, and cell growth. Moreover, alterations in biothiols levels are closely correlated with various health complications triggered by oxidative stress, including cardiovascular disorders, neurodegenerative diseases, and cancer.

Construction of a mitochondrial-targeting near-infrared fluorescent probe for detection of viscosity changes in type 2 diabetes mellitus and nonalcoholic steatohepatitis.

The intracellular viscosity plays a pivotal role as a physicochemical factor and an important indicator of organelles performance. Abnormal changes in subcellular viscosity are often associated with cellular malfunction and various diseases. Nonalcoholic steatohepatitis (NASH) is the most common liver disease related with type 2 diabetes mellitus (T2DM), and both are linked to aberrant mitochondrial viscosity.

An activatable red emitting fluorescent probe for monitoring vicinal dithiol protein fluctuations in a stroke model.

Vicinal dithiol proteins (VDPs) facilitate cellular redox homeostasis, modulate protein synthesis and participate in post-translational modifications through the dynamic equilibrium of dithiol and disulfide bonds. Herein, an activatable red emitting fluorescent probe, VDP-red, is developed for detecting VDPs. With the aid of this probe, we have discovered for the first time a reduction in the levels of reduced VDPs in a stroke mouse model.

Computational insights of excited state intramolecular proton transfer (ESIPT) based fluorescent detection and imaging of γ-glutamytranspeptidase activity.

γ-Glutamytranspeptidase (GGT) is an important tumor biomarker that widely appears in the tumor cells. Therefore, accurate imaging and detection of GGT activity in live cells, serum and pathological cells grasp great importance for the diagnosis, management, and treatment of cancer. Herein, 2-(2-hydroxyl-phenyl)-6-chloro-4-(3H)-quinazolinone (HPQ) is considered as the fluorophore probe for the detection of GGT activity, which is known for the typical mechanism of excited-state intramolecular proton transfer (ESIPT).