Firefly Algorithm-PLS for Synchronous Fluorescence Analysis of Olmesartan and Rosuvastatin in Pharmaceutical and Plasma Samples.

This study presents a validated analytical method for the simultaneous determination of olmesartan and rosuvastatin using synchronous spectrofluorimetry integrated with advanced chemometric modeling. The method addresses the limitations of conventional approaches by combining the firefly algorithm (FA) with partial least squares (PLS) regression to overcome spectral overlap challenges. Experimental conditions were optimized, with ethanol as solvent and 0.

Lithium Disilicate Ceramics Pretreated With Ti: Sapphire fs, Er; Cr: YSGG, and Er: YAG Lasers: A Study on Mechanical Properties, Energy Dispersive Spectroscopy, Color Alterations, and Surface Topography.

To assess the efficiency of Er; Cr: YSGG laser (ECL), Er: YAG laser (EYL), and the Ti: sapphire femtosecond laser (TsFL) in terms of color change (ΔE) measured by a spectrophotometer, surface topography observed through SEM, surface roughness (Ra), and bond strength of Lithium disilicate glass-ceramic (LDC) to resin cement. A total of 84 LDC disks were manufactured and assigned randomly into four groups. Group 1-HFA treated, Group 2 treated with TsFL, Group 3 with EYL, and Group 4 with ECL.

Integrating AI in Healthcare Education: Attitudes of Pharmacy Students at King Khalid University Towards Using ChatGPT in Clinical Decision-Making.

Artificial Intelligence (AI) is transforming healthcare education, offering innovative tools to enhance learning and clinical decision-making. AI-driven platforms like ChatGPT are increasingly integrated into pharmacy education, enabling students to access vast medical knowledge, refine decision-making skills, and simulate clinical scenarios. This study examines pharmacy students' attitudes, knowledge, and practices regarding ChatGPT's use in clinical decision-making, evaluates its perceived benefits and limitations, and identifies factors influencing AI integration in pharmacy education.

Green and Sustainable Determination of Fluconazole Using a Box-Behnken Optimized Zn-CQDs Fluorescence Quenching Approach: Application to Pharmaceutical Formulations and Pharmacokinetic Studies.

In this study, a sensitive and selective analytical method has been developed for the determination of fluconazole, a widely used antifungal agent, using Zn-doped carbon quantum dots as a fluorescent probe. A strong emission from the Zn-CQDs was observed at 440 nm upon excitation at 330 nm, which was quenched in the presence of fluconazole due to a static quenching mechanism, as revealed by Stern-Volmer analysis and thermodynamic studies. The Stern-Volmer constant and the association constant were found to be 6.

Fabrication of extract loaded ZIF-8 nanocomposite for lung cancer treatment, an in vitro assessment.

Medicinal plant-based biocompatible nanomaterials have become increasingly important in the healthcare field. Zeolitic imidazole (ZIF-8) frameworks are fascinating porous crystalline materials that have great attention in the field of drug delivery due to their biodegradability, pH sensitivity to changes in acidic conditions, and chemical durability. The development of ZIF-8 particles loaded with extract (AA) and the evaluation of their anticancer properties are the primary findings of this study.

Targeting Hyperuricemia and NLRP3 Inflammasome in Gouty Arthritis: A Preclinical Evaluation of Allopurinol and Disulfiram Combination Therapy.

Gouty arthritis (GA) is a chronic inflammatory condition characterized by hyperuricemia and NLRP3 inflammasome activation, leading to joint damage and systemic inflammation. Although allopurinol (ALP), a xanthine oxidase inhibitor, effectively lowers serum urate levels, it has limited anti-inflammatory effects. This study investigated whether combining disulfiram (DSF), a known NLRP3 inflammasome inhibitor, with ALP enhances therapeutic outcomes in a rat model of gout.

Development and validation of an environmentally friendly fluorescence quenching method for linagliptin quantification using eosin Y: optimization design of experiment and comparative greenness assessment.

Diabetes management has increasingly relied on dipeptidyl peptidase-4 inhibitors like linagliptin, creating a need for environmentally sustainable analytical methods to replace conventional chromatographic techniques that often involve complex sample preparation, organic solvent usage, and expensive instrumentation. A sensitive and selective "turn-off" fluorescence quenching method was developed and validated for the determination of linagliptin using eosin Y as the fluorescent probe. The spectral characteristics and sensing mechanisms were investigated using Stern-Volmer analysis, Job's method, and thermodynamic studies, revealing a static quenching process driven by the formation of a non-fluorescent 1 : 1 linagliptin-eosin Y complex with a high Stern-Volmer constant ( = 6.

Unveiling Pharmacogenomics Insights into Circular RNAs: Toward Precision Medicine in Cancer Therapy.

Pharmacogenomics is revolutionizing precision medicine by enabling tailored therapeutic strategies based on an individual genetic and molecular profile. Circular RNAs (circRNAs), a distinct subclass of endogenous non-coding RNAs, have recently emerged as key regulators of drug resistance, tumor progression, and therapeutic responses. Their covalently closed circular structure provides exceptional stability and resistance to exonuclease degradation, positioning them as reliable biomarkers and novel therapeutic targets in cancer management.

SASP Modulation for Cellular Rejuvenation and Tissue Homeostasis: Therapeutic Strategies and Molecular Insights.

Cellular senescence regulates aging, tissue maintenance, and disease progression through the Senescence-Associated Secretory Phenotype (SASP), a secretory profile of cytokines, chemokines, growth factors, and matrix-remodeling enzymes. While transient SASP aids wound healing, its chronic activation drives inflammation, fibrosis, and tumorigenesis. This review examines SASP's molecular regulation, dual roles in health and pathology, and therapeutic potential.

Development and Validation of a Simple, Sensitive Fluorescent Method for Eurycomanone Quantification in Tongkat Ali Using Nitrogen-Doped Carbon Quantum Dots and Box-Behnken Design Optimization.

Eurycoma longifolia Jack, commonly known as Tongkat Ali, is a medicinal herb traditionally valued for its aphrodisiac properties, with eurycomanone being its principal bioactive quassinoid. This study introduces a novel, cost-effective method for the sensitive detection of eurycomanone using nitrogen-doped carbon quantum dots (N-CQDs) as a fluorescent probe. The spectral characteristics of the probe were carefully analyzed using UV-vis and fluorescence spectrophotometric techniques, and the sensing mechanism was investigated through Stern-Volmer and thermodynamic studies, revealing a static quenching interaction.

Prevalence of gastrointestinal symptoms after recovery from COVID-19: A questionnaire-based study in the Aseer region.

Background: The coronavirus disease 2019 (COVID-19) pandemic has affected millions of people worldwide, and although it is primarily a respiratory illness, gastrointestinal symptoms have been reported in a significant proportion of patients.

Aim: Prevalence of gastrointestinal symptoms after recovery from COVID-19.

Methodology: A community-based cross-sectional study was conducted in the Aseer region of Saudi Arabia.

Silk Fibroin Nanofibers: Advancements in Bioactive Dressings through Electrospinning Technology for Diabetic Wound Healing.

Background: Non-healing diabetic wounds represent a significant clinical challenge globally, necessitating innovative approaches in drug delivery to enhance wound healing. Understanding the pathogenesis of these wounds is crucial for developing effective treatments. Bioactive dressings and polymeric nanofibers have emerged as promising modalities, with silk biomaterials gaining attention for their unique properties in diabetic wound healing.

Variables that predict hospital stay and the outcome of Fournier gangrene at King Abdulaziz University Hospital: a retrospective study.

Background: The aggressive nature of Fournier gangrene and the associated health issues can result in a more complex clinical course and potentially a longer hospital stay. This study aimed to assess factors that affect the length of hospital stay (LHS) and its relation to the outcome of Fournier gangrene patients.

Methods: A retrospective study was performed at King Abdulaziz University Hospital (KAUH), Saudi Arabia, on patients diagnosed with Fournier gangrene between 2017 and 2023.

Biogenic silver nanoparticle synthesis using orange peel extract and its multifaceted biomedical application.

The aim of this study was to employ an agro-industrial byproduct, specifically Citrus sinensis peels, as a reservoir of polyphenols. The natural chemicals present in C. sinensis peels serve as reducing agents in an environmentally benign method for synthesizing silver nanoparticles (AgNPs).

A Comprehensive Review of Capsaicin and Its Role in Cancer Prevention and Treatment.

This study examines the fundamental chemical mechanisms responsible for capsaicin's advantageous impact on cancer, specifically investigating its influence on several biological processes such as inflammation in cancer metastasis, apoptosis, angiogenesis, and cellular proliferation. This entity's connections with other signaling pathways, including PI3K/AKT, NF-B, and TRPV channels, which have been linked to tumor growth, are thoroughly examined in this work. This study presents a thorough analysis of preclinical studies and clinical trials investigating the efficacy of capsaicin in treating many forms of cancer, such as breast, prostate, colorectal, pancreatic, and others.

Dimensional stability of 3D-printed edentulous and fully dentate hollowed maxillary models over periods of time.

Background: Dental casts made utilising digital workflow are becoming more common because to their speed and cost savings. However, studies on their dimensional accuracy over time with diverse designs are missing.

Objective: The aim of this in vitro study was to assess the dimensional stability of 3D-printed edentulous and fully dentate hollowed maxillary models with 50-micrometer resolution over 1 day, 14 days, and 28 days using surface matching software.

Application of 3 factorial design for loratadine-loaded nanosponge in topical gel formulation: comprehensive in-vitro and ex vivo evaluations.

Loratadine (LoR) is a highly lipophilic and practically insoluble in water, hence having a low oral bioavailability. As it is formulated as topical gel, it competitively binds with the receptors, thus reducing the side-effects. The objective of this study was to prepare LoR loaded nanosponge (LoR-NS) in gel for topical delivery.

Laparo-Endoscopic Single-Site Left Donor Nephrectomy In Patients With Uncommon Renal Vascular Anatomy: Does the Technique Make a Difference?

Background: To compare donor and recipient outcomes in patients with renal artery and ante-aortic renal vein vs unusual renal vascular anatomy undergoing laparoendoscopic single-site donor nephrectomy (LESSDN).

Methods: A retrospective chart review of the comparative study of donor and recipient outcomes of LESSDN in donors with venous abnormality (n = 28, group A), arterial abnormality (n = 74, group B), and standard donors (n = 248, group C).

Results: From September 2016 to August 2022, 350 left LESSDN were performed.

Knowledge, Attitude, and Practices Related to Foot Care Among Diabetic Patients in Tabuk City, Saudi Arabia.

Introduction Diabetic foot ulcer (DFU) is a prevalent complication of diabetes mellitus (DM), affecting approximately 15% of all diabetic patients. This condition poses significant challenges due to its association with major morbidity, mortality, high costs, and diminished quality of life. The incidence of diabetic foot complications among diagnosed diabetes cases is alarming, making it a primary concern in diabetes management.

Association of Oral and General Health with Nutritional Status of Older Adults Attending Two Medical Centers in Riyadh, Saudi Arabia: A Cross-Sectional Study.

Malnutrition could profoundly affect older adults' oral health and quality of life, whereas oral health might, in turn, impact dietary intake and nutritional status. The present study aimed to investigate the association between general and oral health and nutritional status among older adults attending nutrition clinics at two main medical centers in Riyadh, Saudi Arabia. A cross-section study was carried out among adult patients (≥60 years) who attended a geriatric clinic or nutrition clinic at King Khalid University Hospital or King Abdulaziz Medical City, Riyadh.

Metformin HCl-loaded transethosomal gel; development, characterization, and antidiabetic potential evaluation in the diabetes-induced rat model.

Herein we designed, optimized, and characterized the Metformin Hydrochloride Transethosomes (MTF-TES) and incorporate them into Chitosan gel to develop Metformin Hydrochloride loaded Transethosomal gel (MTF-TES gel) that provides a sustained release, improved transdermal flux and improved antidiabetic response of MTF. Design Expert® software (Ver. 12, Stat-Ease, USA) was applied for the statistical optimization of MTF-TES.

Unveiling Nature's potential: Promising natural compounds in Parkinson's disease management.

Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta. Although the exact etiology of PD remains elusive, growing evidence suggests a complex interplay of genetic, environmental, and lifestyle factors in its development. Despite advances in pharmacological interventions, current treatments primarily focus on managing symptoms rather than altering the disease's underlying course.

Trastuzumab-Mediated Cardiotoxicity and Its Preventive Intervention by Zingerone through Antioxidant and Inflammatory Pathway in Rats.

Trastuzumab (TZB) is a new medicine, used to treat cancers of the breast and stomach. However, the cardiotoxic potential of this drug edges out its clinical advantages. The present study was designed to find out the effect of zingerone against trastuzumab-mediated cardiotoxicity in rats.

An Insight into Codon Pattern Analysis of Autophagy Genes Associated with Virus Infection.

Introduction: Apoptosis and autophagy are the two fundamental processes involved in maintaining homeostasis, and a common stimulus may initiate the processes. Autophagy has been implicated in various diseases, including viral infections. Genetic manipulations leading to altered gene expression might be a strategy to check virus infection.

Therapeutic Potential of Capsaicin against Cyclophosphamide-Induced Liver Damage.

Cyclophosphamide (CPM) is a classical alkylating agent used in different cancer chemotherapy regimens and is restricted due to severe adverse effects, including hepatotoxicity. Natural or plant-derived antioxidants such as capsaicin were utilized in this study to examine the hepatoprotective benefits against cyclophosphamide-induced hepatotoxicity. The rats were divided into five groups: a normal control group, a toxic group (CPM), an intraperitoneal injection of a single dose of 200 mg/kg b.