Publications by authors named "Sasi Mudumba"
The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG) batches. We found that most COVID-19 patients developed anti-SARS-CoV-2 IgG antibodies before IgM.
View Article and Find Full Text PDFObjectives: Our aim was to evaluate systemic lupus erythematosus (SLE) disease activity and SARS-CoV-2-specific immune responses after BNT162b2 vaccination.
Methods: In this prospective study, disease activity and clinical assessments were recorded from the first dose of vaccine until day 15 after the second dose in 126 patients with SLE. SARS-CoV-2 antibody responses were measured against wild-type spike antigen, while serum-neutralising activity was assessed against the SARS-CoV-2 historical strain and variants of concerns (VOCs).
Humoral immune responses are typically characterized by primary IgM antibody responses followed by secondary antibody responses associated with immune memory and composed of IgG, IgA, and IgE. Here, we measured acute humoral responses to SARS-CoV-2, including the frequency of antibody-secreting cells and the presence of SARS-CoV-2-specific neutralizing antibodies in the serum, saliva, and bronchoalveolar fluid of 159 patients with COVID-19. Early SARS-CoV-2-specific humoral responses were dominated by IgA antibodies.
View Article and Find Full Text PDFArticle Synopsis
- - The study examines two subtypes of juvenile idiopathic inflammatory myopathy (JIIM) associated with anti-MDA5 autoantibodies, finding they have distinct clinical features compared to other JIIM types, such as more arthritis and skin ulcerations but less severe muscle involvement.
- - Researchers analyzed 64 patients from French pediatric rheumatology centers, using muscle biopsies and measuring IFNα serum protein levels to assess disease status and treatment outcomes.
- - The findings indicate that systemic IFNα plays a significant role in the pathology of JIIM with anti-MDA5 autoantibodies, suggesting it could be a target for treatment in severe cases.
Article Synopsis
- A new technology, photonic ring immunoassay (PRI), allows for the simultaneous detection of 12 autoantibodies in whole blood within 15 minutes.
- In a study with 235 patients, PRI demonstrated over 91% overall agreement with standard lab tests, showing high sensitivity and specificity.
- Additionally, tests on 199 normal blood donors showed specificities exceeding 97.5%, indicating PRI's potential for quick point-of-care testing and timely results for doctors.
Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time.
J Immunol Methods
September 2017
Photonic ring resonance is a property of light where in certain circumstances specific wavelengths are trapped in a ring resonator. Sensors based on silicon photonic ring resonators function by detecting the interaction between light circulating inside the sensor and matter deposited on the sensor surface. Binding of biological material results in a localized change in refractive index on the sensor surface, which affects the circulating optical field extending beyond the sensor boundary.
View Article and Find Full Text PDFAdvantages of multiplex proteomics in clinical immunology: the case of rheumatoid arthritis: novel IgXPLEX™: planar microarray diagnosis.
Clin Rev Allergy Immunol
August 2011
Article Synopsis
- Clinical multiplex diagnostic proteomics aims to enhance patient outcomes through advanced testing methods for disease prognosis, diagnosis, and treatment monitoring using data from body fluids.
- Despite its potential, challenges such as complexity of clinical samples and interference from high-abundance proteins must be addressed to improve accuracy.
- An example of success in this field is the development of a sensitive rheumatoid arthritis assay, which can simultaneously measure multiple disease markers with high sensitivity (82.5%) and specificity (97.7%) in a small serum sample.