Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase.

Babesia bigemina, a tick-borne protozoan parasite, is one of the main causative agents of bovine babesiosis, a disease with significant economic impact on the cattle industry. One of the key enzymes involved in the parasite's metabolism is lactate dehydrogenase (LDH), which plays an essential role in the anaerobic glycolytic pathway by catalysing the conversion of pyruvate to lactate. In this study, B.

Investigation of potential KRASG12D inhibitors: a comparative study between MRTX1133 and natural compounds via computational structural biology approaches.

Objective: The RAS family, comprising KRAS, NRAS, and HRAS, plays a pivotal role in oncogenesis, dynamically regulating cellular processes through intricate cycling between active and inactive states. Despite recent advancements, direct therapeutic targeting of RAS proteins has proven challenging. Targeting KRASG12D with natural compounds offers a unique therapeutic potential, leveraging the structural diversity and bioactivity of natural compounds.

Exploring novel inhibitors for Babesia bigemina lactate dehydrogenase: a computational structural biology perspective.

Babesia bigemina is an apicomplexan parasite responsible for causing "Texas fever" in bovines. Current treatments for bovine babesiosis are hindered by several limitations, including toxicity, insufficient efficacy in eliminating the parasite, and the potential for resistance development. A promising approach to overcome these challenges is the identification of compounds that specifically target essential metabolic pathways unique to the parasite.

Effects of foliar iron oxide nanoparticles (FeO) application on photosynthetic parameters, distribution of mineral elements, magnetic behaviour, and photosynthetic genes in tomato (Solanum lycopersicum var. cerasiforme) plants.

This study aims to examine the effect of foliar magnetic iron oxide (FeO) nanoparticles (IONP) application on the physiology, photosynthetic parameters, magnetic character, and mineral element distribution of cherry tomatoes (Solanum lycopersicum var. cerasiforme). The IONP suspension (500 mg L) was sprayed once (S1), twice (S2), thrice (S3), and four times (S4) a week on seedlings.

Genome-wide identification of barley MCs (metacaspases) and their possible roles in boron-induced programmed cell death.

Developmental processes and stress-induction activate many key proteins in plants such as metacaspase which regulate programmed cell death (PCD). In this study, identification of barley metacaspases and their possible roles upon boron (B)-induction was investigated by using in silico and wet-lab methods. Genome-wide analysis revealed that barley genome harbor ten metacaspases which divided into three groups: Type-I, -I* and -II.