Copper homeostasis in Streptococcus and Neisseria: Known knowns and unknown knowns.

Our research group studies copper (Cu) homeostasis in Streptococcus and Neisseria, with a current focus on species that colonise the human oral cavity. Our early ventures into this field very quickly revealed major differences between well-characterised Cu homeostasis systems in species with well-known pathogenic potential and the uncharacterised systems in species that are considered as components of the normal healthy human microflora. In this article, we summarise the known and predicted mechanisms of Cu homeostasis in Streptococcus and Neisseria.

Generation of Multispecies Oral Bacteria Biofilm Models.

It is well-recognized that oral biofilms that occur in health and disease have a polymicrobial composition, though these are poorly reflected in the literature, with many studies focussing on simple mono-species biofilm model systems. The utility of polymicrobial biofilm model systems is that they more accurately reflect the oral cavity and allow researchers to ask relevant questions in basic science studies, pharmaceutical screening, and investigating inflammatory interactions. Here we describe the detailed methodology of how to sequentially construct and maintain polymicrobial biofilm models pertinent to caries, periodontal disease, and denture stomatitis.