Establishing an porcine skin model to investigate the effects of broad-spectrum antiseptic on viable skin microbial communities.

Unlabelled: Incomplete antiseptic efficacy against potentially pathogenic microbial taxa places some patients at disproportionate risk for developing a surgical site infection. Laboratory models capable of interrogating the effects of antiseptics on the skin and its complex microbial communities are desperately needed to improve and better tailor antiseptic formulations. This work aims to establish an porcine skin model to explore the impact of topical antiseptics on complex skin microbial communities and superficial skin lipids.

Respiratory rate and breathing pattern in dogs and cats in intensive care units and the value of camera surveillance.

Objectives: Information on respiratory rate and breathing pattern of dogs and cats in intensive care units is limited. This study aimed to evaluate whether respiratory rate differed in dogs and cats in the intensive care unit when monitored via camera surveillance (Cam+) compared to traditional cage-side monitoring (Cam-) at various times during the intensive care unit stay, between animals treated for left-sided congestive heart failure, respiratory diseases and other conditions, and if associations existed between respiratory rate and breathing pattern.

Materials And Methods: Privately owned dogs (n = 41) and cats (n = 60) treated and monitored in the intensive care units of two small animal hospitals were enrolled in this study.

Establishing an ex vivo porcine skin model to investigate the effects of broad spectrum antiseptic on viable skin microbial communities.

Incomplete antiseptic efficacy against potentially pathogenic microbial taxa places some patients at disproportionate risk for developing a surgical site infection. Laboratory models capable of interrogating the effects of antiseptics on the skin and its complex microbial communities are desperately needed to improve and better tailor antiseptic formulations. This work aims to establish an ex vivo porcine skin model to explore the impact of topical antiseptics on complex skin microbial communities and superficial skin lipids.

Still not sterile: viability-based assessment of the skin microbiome following pre-surgical application of a broad-spectrum antiseptic reveals transient pathogen enrichment and long-term recovery.

Broad-spectrum antiseptics such as chlorhexidine gluconate (CHG) have widespread use as pre-surgical tools to lower skin microbial burden and reduce the risk of surgical site infection. However, the short- and long-term effects of CHG on healthy skin microbial communities remain undefined due to the confounding effects of CHG binding with persistent bacterial DNA on the skin surface. Here, we aim to accurately characterize the immediate and long-term impact of pre-surgical preparation with CHG-based antiseptics on the human skin microbiome.

Usual suspects meet mission impossible: Nutrient losses and effects of mitigation measures on a coastal catchment in the Baltic Sea region.

Eutrophication of coastal areas is a global problem. A full-scale coastal remediation project was initiated in Björnöfjärden bay in the Stockholm archipelago in 2011. Measures to reduce external nutrient inputs from the surrounding catchment (15 km) targeted agriculture, on-site wastewater treatment facilities, and horse keeping.