Publications by authors named "Ruchi B Sheth"
Levels of protein translation by ribosomes are governed both by features of the translation machinery as well as sequence properties of the mRNAs themselves. We focus here on a striking three-nucleotide periodicity, characterized by overrepresentation of GCN codons and underrepresentation of G at the second position of codons, that is observed in Open Reading Frames (ORFs) of mRNAs. Our examination of mRNA sequences in Saccharomyces cerevisiae revealed that this periodicity is particularly pronounced in the initial codons-the ramp region-of ORFs of genes with high protein expression.
View Article and Find Full Text PDFTranscriptomic Analysis of Octanoic Acid Response in Using RNA-Sequencing.
Stephen M Lanno
,
Sara M Gregory
,
Serena J Shimshak
,
Maximilian K Alverson
,
Kenneth Chiu
,
Ruchi B Sheth
G3 (Bethesda)
December 2017
Article Synopsis
- The dietary specialist fruit fly has adapted to thrive on the toxic fruit of its host plant, which contains high levels of octanoic acid (OA).
- Previous research using RNA interference (RNAi) indicated that certain genes are crucial for resistance to OA, revealing that multiple regions of the genome influence this trait.
- In this study, RNA-sequencing was used to identify 104 differentially expressed genes in response to OA exposure, highlighting new candidate genes and their roles in cuticle development and immune responses that may contribute to OA resistance.