Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon.

Grasses form morphologically derived, four-celled stomata, where two dumbbell-shaped guard cells (GCs) are flanked by two lateral subsidiary cells (SCs). This innovative form enables rapid opening and closing kinetics and efficient plant-atmosphere gas exchange. The mobile bHLH transcription factor MUTE is required for SC formation in grasses.

Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases.

Hydroxylation of prolines to 4-trans-hydroxyproline (Hyp) is mediated by prolyl-4 hydroxylases (P4Hs). In plants, Hyps occur in Hydroxyproline-rich glycoproteins (HRGPs), and are frequently -glycosylated. While both modifications are important, for cell wall stability, they are undesired in plant-made pharmaceuticals.

Opposite polarity programs regulate asymmetric subsidiary cell divisions in grasses.

Grass stomata recruit lateral subsidiary cells (SCs), which are key to the unique stomatal morphology and the efficient plant-atmosphere gas exchange in grasses. Subsidiary mother cells (SMCs) strongly polarise before an asymmetric division forms a SC. Yet apart from a proximal polarity module that includes PANGLOSS1 (PAN1) and guides nuclear migration, little is known regarding the developmental processes that form SCs.

Morphology made for movement: formation of diverse stomatal guard cells.

Stomata constantly open and close to optimize gas exchange. While the genetic programme guiding early development is well described, the formation of functional guard cells remains enigmatic. This review highlights recent findings on the developmental and morphogenetic processes shaping this essential and morphologically diverse cell type in Arabidopsis and grasses.