A Machine Learning-Enabled SERS Sensor: Multiplex Detection of Lipopolysaccharides from Foodborne Pathogenic Bacteria.

Foodborne pathogenic bacteria pose a global threat to public health, causing dangerous and expensive outbreaks. While therapeutics are being developed to target antibiotic-resistant and foodborne pathogenic bacteria, approaches to minimize their spread are also crucial. As estimated by the World Health Organization, each year around 600 million cases of foodborne illnesses are reported, with over 420,000 deaths annually.

Progress and limitations in reactive oxygen species quantitation.

Reactive oxygen species (ROS) are a set of oxygen- and nitrogen-containing radicals. They are produced from a wide range of sources. In biological contexts, cellular stress leads to an overproduction of ROS, which can lead to genetic damage and disease development.

Dual Fragmentation Isobaric Tags for Metabolomics.

Isobaric tags typically leverage an a1 type fragmentation to produce constant mass reporter ions. While this motif allows for efficient reporter formation, isobaric tags lack structural diversity, which limits the number and type of isotopes that are synthetically available. Presented here are two examples of dual fragmentation isobaric tagging.

Isobaric 6-plex and tosyl dual tagging for the determination of positional isomers and quantitation of monounsaturated fatty acids using rapid UHPLC-MS/MS.

Isobaric labelling of fatty acids is complicated by chromatographic co-elution of double bond isomers. This produces contaminated spectra which can mask important biological changes. Here two derivatization strategies are combined to improve throughput and produce MS reporters which change mass depending on double bond position.