Molecular-Level Insights into Recalcitrant Ozonation Products from Effluent Organic Matter.

Wastewater ozonation is commonly employed to enhance the subsequent biodegradation of effluent organic matter (EfOM) and contaminants of concern. However, there is evidence suggesting the formation of recalcitrant ozonation products (OPs) from EfOM. To investigate the biodegradability of OPs we conducted batch biodegradation experiments using wastewater effluent ozonated with mass-labeled (O) ozone.

Post column infusion of an internal standard into LC-FT-ICR MS enables semi-quantitative comparison of dissolved organic matter in original samples.

Ultrahigh resolution mass spectrometry hyphenated with liquid chromatography (LC) is an emerging tool to explore the isomeric composition of dissolved organic matter (DOM). However, matrix effects limit the potential for semi-quantitative comparison of DOM molecule abundances across samples. We introduce a post-column infused internal standard (PCI-IS) for reversed-phase LC-FT-ICR MS measurements of DOM and systematically evaluate matrix effects, detector linearity and the precision of mass peak intensities.

Phase II stanozolol metabolism study using the zebrafish water tank (ZWT) model.

Stanozolol (STAN) is an androgen anabolic steroid often misused in sports competitions and prohibited at all times by the World Anti-Doping Agency (WADA). It can be long term detected by the analysis of human urine for traces of intact glucuronide metabolites. The Zebrafish Water Tank (ZWT) experimental setup can produce phase I STAN metabolites.