Publications by authors named "Raj Narayan Yadav"
Purpose: The present study aimed to find out the most prevalent lineage of Mycobacterium tuberculosis (MTB) and analysis of their mutation profile conferring to drug resistance at National Reference Laboratory (NRL) in New Delhi.
Methods: A total 212 liquid culture MTB isolates of presumptive multidrug resistance tuberculosis (MDR-TB) patients were selected at NRL and subjected to whole genome sequencing (Illumina Miseq, USA). The genome sequencing data of 194 samples was successfully obtained in fastq files from instrument and analysed by Mykrobe predictor for lineage and mutation identification conferring drug resistance.
Background: Nontuberculous mycobacteria (NTM) infections are an emerging global health concern with increasing incidence. Conventional identification methods for NTM species in clinical settings are prone to errors. This study evaluates a newer method, polymerase chain reaction-restriction enzyme analysis (PCR-REA) of the rpoB gene, for NTM species identification.
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- A rapid drug susceptibility test is crucial for combating drug-resistant tuberculosis, with research exploring next generation sequencing (NGS) as a replacement for traditional testing methods.
- This study assessed the effectiveness of a genome analysis tool, AAICare®-TB, for predicting drug resistance in Mycobacterium tuberculosis compared to two other tools, TB-Profiler and Mykrobe, using whole genome sequences from 120 strain isolates.
- AAICare®-TB successfully predicted drug resistance for multiple first and second-line tuberculosis drugs in 93 samples, aligning with WHO guidelines on mutation categorization.
Introduction: Tuberculosis (TB) remains a deadliest infectious disease. Lack of rapid test with low cost is one of the important challenges to eradicate the TB. The objective of the study was to analyze the laboratory costs of conventional and newer molecular tests, for diagnosis of presumptive multidrug-resistant TB (MDR-TB) patients.
View Article and Find Full Text PDFBackground: Recently, moxifloxacin (MFX)-resistant results of Mycobacterium tuberculosis (Mtb) obtained by GenoType MTBDRsl (second-line line probe assay [SL-LPA]) have been stratified to determine their resistance level; however, its accuracy has not been well studied. Therefore, the study aimed to evaluate the diagnostic accuracy of SL-LPA, with phenotypic drug susceptibility testing (pDST) and whole-genome sequencing (WGS) for the detection of MFX-resistant Mtb and their resistance level.
Methods: A total of 111 sputum samples were subjected to SL-LPA according to the diagnostic algorithm of the National Tuberculosis Elimination Program.
Multidrug-resistant and extensively drug-resistant strains in geriatrics: An analysis and its implications in tuberculosis control.
J Clin Tuberc Other Mycobact Dis
May 2022
Objective: This study aimed to analyze the trends of tuberculosis (TB) disease, drugs susceptibility patterns in geriatric TB over a period of three years (from 2010 to 2012).
Materials & Methods: In this study, laboratory data on diagnosis of geriatric tuberculosis suspected patients (age ≥60 years) was analyzed retrospectively at National Reference Laboratory (NRL).
Results: Among 12,140 geriatric TB suspects, 1621 (13%) were acid-fast bacillus (AFB) smear-positive and 10,519 (87%) were smear-negative.
Article Synopsis
- Toll-like receptors (TLRs) are crucial in the innate immune system by recognizing pathogens, and mutations in TLR genes can affect susceptibility to infections.
- The study included a variety of participants, including HIV-positive patients with different conditions related to tuberculosis (TB), and analyzed genetic differences linked to TLR4.
- Results showed significant differences in TLR4 gene frequencies among various groups, suggesting that specific TLR polymorphisms are linked to a higher risk of developing active TB in patients with HIV.
Background: The emergence of drug-resistant tuberculosis (TB), is a major menace to cast off TB worldwide. Line probe assay (LPA; GenoType MTBDRplus ver. 2) and Xpert MTB/RIF assays are two rapid molecular TB detection/diagnostic tests.
View Article and Find Full Text PDFPulmonary tuberculosis still remains a major communicable disease worldwide. In 2013, 9 million people developed TB and 1.5 million people died from the disease.
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