Genomic hybrid capture assay to detect : an application to diagnose neuroborreliosis in horses.

Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the flagellin gene was negative. However, we enriched DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified in the CSF of the horse, confirming a diagnosis of neuroborreliosis.

Evaluation and management of the recumbent adult horse.

Evaluation and management of recumbent horses are challenging. Familiarity with disorders that can result in recumbency will facilitate more rapid diagnosis and more appropriate formulation of a prognosis. With rapid and appropriate diagnosis, appropriate treatment can be pursued and, with good nursing care, can result in a favorable outcome.

Efficacy of sublingual administration of detomidine gel for sedation of horses undergoing veterinary and husbandry procedures under field conditions.

Objective: To determine whether sublingual detomidine gel administration to horses would be effective in providing an appropriate degree of sedation and restraint to facilitate completion of veterinary and husbandry procedures under field conditions.

Design: Multicenter, prospective, randomized, blinded, placebo-controlled clinical study.

Animals: 270 client-owned horses known to require sedation or strong restraint to enable veterinary and husbandry procedures to be performed.

Serum opsonization capacity, phagocytosis, and oxidative burst activity in neonatal foals in the intensive care unit.

Background: Phagocytic activity of neonatal foals has been reported to be similar to that of adult horses, but serum opsonization capacity develops with age and may be further altered when opsonins are consumed during infection.

Hypothesis: Phagocytosis, oxidative burst activity, and serum opsonization capacity in neonatal foals admitted to an intensive care unit are reduced in comparison with control foals.

Animals: Blood samples were collected from hospitalized neonatal foals and from control foals.

Serum gamma glutamyl transferase activity in horses with right or left dorsal displacements of the large colon.

The purpose of this study was to test the hypothesis that horses with right dorsal displacement of the large colon (RDDLC) have elevations in serum gamma glutamyl transferase (GGT) activity when compared with horses with left dorsal displacement of the large colon (LDDLC). Medical records from 37 horses with RDDLC and 48 horses with LDDLC were reviewed. Horses were included for study if the RDDLC or LDDLC was confirmed by exploratory laparotomy or postmortem examination and if a serum GGT measurement was obtained within 24 hours before surgery.