Genotype-Driven Proteomic Diversity in Macadamia Nuts: Implications for Allergenicity, Nutritional Quality, and Breeding Strategies.

Macadamia nuts are valued for their nutritional content, yet little is known about the factors that affect nut quality or the genetic contributions of parental lines. This study optimized a protein extraction protocol for lipid-rich macadamia nuts and applied SDS-PAGE and mass spectrometry-based proteomics to characterize nuts with known parental origin. A total of 431 high-confidence proteins were identified, with seed storage proteins (SSPs), primarily vicilin- and legumin-like globulins, comprising nearly 50% of total protein abundance.

Addendum to Dopamine Cell-Based Replacement Therapies.

Since the publication of our article "Dopamine Cell-Based Replacement Therapies" (Kayhanian and Barker. 2025. doi:10.

LINE-1 retrotransposons mediate cis-acting transcriptional control in human pluripotent stem cells and regulate early brain development.

Long interspersed nuclear element 1 (L1) retrotransposons represent a vast source of genetic variability. However, mechanistic analysis of whether and how L1s contribute to human developmental programs is lacking, in part due to the challenges associated with specific profiling and manipulation of human L1 expression. Here, we show that thousands of hominoid-specific L1 integrants are expressed in human induced pluripotent stem cells and cerebral organoids.

Spatially-resolved quantification of erosion and swelling of tablets in a paddle dissolution apparatus with integrated optical coherence tomography.

Disintegration and dissolution play a key role in drug release from oral immediate-release products. An improved understanding of these processes, the impact of process parameters and the critical material attributes are required to develop robust formulations and manufacturing processes. This study demonstrates an in-situ disintegration and dissolution monitoring system capable of capturing quantitative swelling and erosion data in a paddle dissolution apparatus.