Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids.

Angelman syndrome is a neurodevelopmental disorder caused by (epi)genetic lesions of maternal UBE3A. Research has focused largely on the role of UBE3A in neurons due to its imprinting in that cell type. Yet, evidence suggests there may be broader neurodevelopmental impacts of UBE3A dysregulation.

Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development.

Human cerebral organoids are readily generated from human embryonic stem cells and human induced pluripotent stem cells and are useful in studying human neurodevelopment. Recent work with human cerebral organoids have explored the creation of different brain regions and the impacts of soluble and mechanical cues. Matrigel is a gelatinous, heterogenous mixture of extracellular matrix proteins, morphogens, and growth factors secreted by Engelbreth-Holm-Swarm mouse sarcoma cells.

An interesting case of intracerebral MRSA.

This article describes a patient whose intracerebral MRSA may have been caused by a spider bite to the thigh 2 months earlier. The patient's headache, nausea, and vomiting initially were thought to be caused by a high-grade glioma. Most brain abscesses have otogenic sources and are not from distant hematogenous spread.

Understanding Guillain-Barré syndrome.

Guillain-Barré syndrome (GBS) is a rapidly progressive peripheral neuropathy that most commonly presents with ascending symmetrical weakness and diminished or absent deep tendon reflexes. Because weakness may affect the diaphragm and cause respiratory distress, 10% to 30% of patients require mechanical ventilation. Symptoms progress and peak about 4 weeks after onset.